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  • How to design gRNA and gRNA expression vector

    How to design gRNA and gRNA expression vector The expression vector can drive high-level constitutive transcription of a user-selected gRNA sequence to achieve highly efficient CRISPR targeting when used in conjunction with Cas9 nuclease. A routine gRNA expression vector system wo…

  • Reporter KI cell model application

    Reporter KI cell model application Since it was developed in 2012, this gene-editing tool has revolutionized biology research, making it easier to study disease and faster to discover drugs. The CRISPR/Cas9 system has become synonymous with genome editing in today’s world due to…

  • Gene knockout cell line: How to design gRNAs and avoid protein residues?

    Gene knockout cell line: How to design gRNAs and avoid protein residues? Genome editing is a dream method for accurately and precisely recognizing and locating a specific sequence within a genome, and then altering the targeted sequences for various purposes. The CRISPR/Cas9 syste…

  • How to study point mutation with gene editing in cell lines

    How to study point mutation with gene editing in cell lines What is point mutation? A point mutation is specifically when only one nucleotide base is changed in some way. Types of point mutations: Point mutation can be classified based on their location and function. Based on loca…

  • [Research Highlight] TXNIP positively regulates the autophagy and apoptosis in the rat müller cell o

    [Research Highlight] TXNIP positively regulates the autophagy and apoptosis in the rat müller cell of diabetic retinopathy Diabetic retinopathy (DR) is a microvascular complication of diabetes, threatening the vision of working-age and aged population worldwide, which is preventa…


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