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  • "Molecular Biologist Toolbox" E. coli with CRISPR form a new pace in biological studies

    "Molecular Biologist Toolbox" E. coli with CRISPR form a new pace in biological studies By Dr. ASAD UZZAMAN The microorganism Escherichia coli (E.coli) has a long history in the biotechnology industry and is still the microorganism of choice for most gene manipulation research. Fa…

  • CT26.WT cell line, a magic route for colon cancer research & therapeutics

    CT26.WT cell line, a magic route for colon cancer research therapeutics By Dr. ASAD UZZAMAN Murine CT26 (Colon Tumor #26) cells were developed in 1995 by exposing BALB/c mice to N-nitroso-N-methylurethane (NMU) that form a rapid-growing grade IV carcinoma. These cells are easily i…

  • Knockout cell pool boost researcher confidence in gene function study

    Knockout cell pool boost researcher confidence in gene function study CRISPR is a revolutionary genome-editing technology and it can be used to edit genes and, as such, will likely change the world. Although it’s a relatively recent discovery, CRISPR quickly becomes a standard l…

  • How to study protein kinase by CRISPR technology?

    How to study p rotein kinase by CRISPR technology? Protein kinases (PK) and their opponents, phosphatases, have been crucial to many scientists. Approximately 2% of the human genome encodes for PKs known as human “kinome”, consisting of 518 protein kinases and their variants. …

  • ARPE-19, Genome Surgery and Vision Science, A combo for tackling blindness

    ARPE-19 cell line has a visually normal karyotype and expresses RPE-specific markers, the retinal pigment epithelium-specific 65kDa protein (RPE65), and cellular retinaldehyde-binding protein (CRALBP), as has been shown at the mRNA and protein levels, respectively.


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