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  • Combination of HCT116 cells and CRISPR/Cas9: A perfect group for colorectal cancer treatment research

    Combination of HCT116 cells and CRISPR/Cas9: A perfect group for colorectal cancer treatment research 1. Cell information and applications 1.1 Cell information HCT 116 was isolated from a 48 year old male patient with colorectal cancer in 1979 by M. brattain et al. The cells can f…

  • Application of KO cells

    Application of KO cells CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats), the term refers to a series of repetitive patterns in the DNA of bacteria and archaea that were extensively studied by Spanish scientist Francis Mojica in the ‘90s. These patterns are …

  • How to construct a KO cell line

    How to construct a KO cell line CRISPR/Cas9 technology enables the rapid generation of loss‐of‐function mutations in a targeted gene and changed the way of scientists approach their research. A single cell is a harbor of mutations that can be used to establish a new cell line,…

  • How to design gRNA and gRNA expression vector

    How to design gRNA and gRNA expression vector The expression vector can drive high-level constitutive transcription of a user-selected gRNA sequence to achieve highly efficient CRISPR targeting when used in conjunction with Cas9 nuclease. A routine gRNA expression vector system wo…

  • Reporter KI cell model application

    Reporter KI cell model application Since it was developed in 2012, this gene-editing tool has revolutionized biology research, making it easier to study disease and faster to discover drugs. The CRISPR/Cas9 system has become synonymous with genome editing in today’s world due to…


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