HCT 116-CAS9 cell line

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location： Home > Products > Cas9 Stable Cell Lines > HCT 116-CAS9

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HCT 116-CAS9 Order

HCT 116-CAS9 cell line
Catalog#
YC-C004-Cas9-H
Size
1*10^6
Transfection Culture Medium
Transfection Culture Medium-5A
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Datasheet
HCT 116-CAS9 cell line
Order

Detailed Product Information

Product category
Cas9 Stable Cell Lines
cell name
HCT 116-CAS9
Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Expression gene
Cas9
Resistance gene
hygro
Construction method
Lentivirus
Mycoplasma test
negative
Culture method
90%McCOY's 5A+10% FBS
Description
Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the HCT 116 cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a HCT 116-CAS9 cell line that can maintain long-term, stable and high expression.
Product Validation Data（RT-QPCR）
Sample Name Target Name Cт Mean ΔCт
HCT 116-CAS9 Cas9 16.68340492 -0.45818520
HCT 116-CAS9 h-actin-B 17.14159012
HCT 116 Cas9 33.37912369 15.92828751
HCT 116 h-actin-B 17.45083618
Cutting Efficiency Validation
HCT 116-CAS9 cell line Product Validation Data（RT-QPCR）
Note: The above figure shows the sequencing peaks of the HCT 116-CAS9 stable cell pool which is electroporated by NONO gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:HCT 116		 STR Info (Cell bank)
Cell Line:HCT 116
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 10111011
D13S317 10121012
D7S820 11121112
D16S539 11131113
VWA 17221717
TH01 8989
AMEL XXXY
TPOX 8889
CSF1PO 710710
D12S391 1721
FGA 1823
D2S1338 1616
D21S11 2930
D18S51 1617
D8S1179 1214
D3S1358 1219
D6S1043 1313
PENTAE 1314
D19S433 1213
PENTAD 913
D1S1656 1214
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

  • Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
    01
  • High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
    02
  • High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
    03
HCT 116-CAS9 cell line
HCT 116-CAS9
Catalog#
YC-C004-Cas9-H
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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