IL6ST Knockout cell line (Hep G2)

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IL6ST Knockout cell line (Hep G2) Order

IL6ST Knockout cell line (Hep G2)
Catalog#
YKO-HT1502
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
IL6ST Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
IL6ST Knockout cell line (Hep G2)
Species
Human
Gene Name
IL6ST
Gene ID
3572
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 7 (based on transcript IL6ST-204)
Validation: PCR+Sanger Sequencing
IL6ST Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the IL6ST gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, IL6ST gene knockout IL6ST Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
IL6ST Knockout cell line (Hep G2)
IL6ST Knockout cell line (Hep G2)
Catalog#
YKO-HT1502
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • IL6ST Knockout cell line (THP-1)

    IL6ST Knockout cell line (THP-1)
      inquiry   More details

    Organism: Human
    Gene Name: IL6ST
    Gene ID: 3572
    Size: 1*10^6
    Catalog#: YKO-HT0427
    YKO-HT0427(THP-1)
  • IL6ST Knockout cell line (HeLa)

    IL6ST Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: IL6ST
    Gene ID: 3572
    Size: 1*10^6
    Catalog#: YKO-HS3628
    YKO-HS3628(HeLa)
  • IL6ST Knockout cell line (HCT 116)

    IL6ST Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: IL6ST
    Gene ID: 3572
    Size: 1*10^6
    Catalog#: YKO-HS2505
    YKO-HS2505(HCT 116)

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Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • IL6ST Knockout cell line (THP-1)

    IL6ST Knockout cell line (THP-1)

    Organism: Human
    Gene Name: IL6ST
    Gene ID: 3572
    Size: 1*10^6
    Catalog#: YKO-HT0427
      inquiry   More details
  • IL6ST Knockout cell line (HeLa)

    IL6ST Knockout cell line (HeLa)

    Organism: Human
    Gene Name: IL6ST
    Gene ID: 3572
    Size: 1*10^6
    Catalog#: YKO-HS3628
      inquiry   More details
  • IL6ST Knockout cell line (HCT 116)

    IL6ST Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: IL6ST
    Gene ID: 3572
    Size: 1*10^6
    Catalog#: YKO-HS2505
      inquiry   More details

IL6ST Knockout cell line (Hep G2) Order

IL6ST Knockout cell line (Hep G2)
Catalog#
YKO-HT1502
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
IL6ST Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
IL6ST Knockout cell line (Hep G2)
Species
Human
Gene Name
IL6ST
Gene ID
3572
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 7 (based on transcript IL6ST-204)
Validation: PCR+Sanger Sequencing
IL6ST Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the IL6ST gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, IL6ST gene knockout IL6ST Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
IL6ST Knockout cell line (Hep G2)
IL6ST Knockout cell line (Hep G2)
Catalog#
YKO-HT1502
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • IL6ST Knockout cell line (THP-1)

    IL6ST Knockout cell line (THP-1)
      inquiry   More details

    Organism: Human
    Gene Name: IL6ST
    Gene ID: 3572
    Size: 1*10^6
    Catalog#: YKO-HT0427
    YKO-HT0427(THP-1)
  • IL6ST Knockout cell line (HeLa)

    IL6ST Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: IL6ST
    Gene ID: 3572
    Size: 1*10^6
    Catalog#: YKO-HS3628
    YKO-HS3628(HeLa)
  • IL6ST Knockout cell line (HCT 116)

    IL6ST Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: IL6ST
    Gene ID: 3572
    Size: 1*10^6
    Catalog#: YKO-HS2505
    YKO-HS2505(HCT 116)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • IL6ST Knockout cell line (THP-1)

    IL6ST Knockout cell line (THP-1)

    Organism: Human
    Gene Name: IL6ST
    Gene ID: 3572
    Size: 1*10^6
    Catalog#: YKO-HT0427
      inquiry   More details
  • IL6ST Knockout cell line (HeLa)

    IL6ST Knockout cell line (HeLa)

    Organism: Human
    Gene Name: IL6ST
    Gene ID: 3572
    Size: 1*10^6
    Catalog#: YKO-HS3628
      inquiry   More details
  • IL6ST Knockout cell line (HCT 116)

    IL6ST Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: IL6ST
    Gene ID: 3572
    Size: 1*10^6
    Catalog#: YKO-HS2505
      inquiry   More details

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