Membrane protein gRNA library

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location： Home > Products > CRISPR-iScreen™ Library Plasmid > Membrane protein gRNA library

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Membrane protein gRNA library

Membrane protein gRNA library

Catalog#： LIBR-H006-P002 LIBR-H006-P100 LIBR-H006-P200 LIBR-H006-P500

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Detailed Product Information Recommended Companion Products
The CRISPR activation library targets 6,213 human membrane protein genes with a total of 58,570 gene activation plasmid vectors, of which 9 different gRNA vectors designed for each gene, in addition to 500 control vectors targeting intergenic sequences. The library uses pKVL2-U6gRNA_SAM(BbsI)-PGKpuroBFP-W as the backbone, which is a triple plasmid system that expresses only gRNA, while the dCas9-VP64 and MS2-P65-HSF1 genes are on two other different vectors that need to be used in combination.
Product Name
Membrane protein gRNA library
Species
Human
Library Type
Activation Library
Plasmid System
Triple-plasmid System
Virus Packaging System
3rd Lentivirus Packaging System
Targeted Genes
6213
gRNA Number
58570
Non-targeting gRNA Number
500
Selection Marker
Puro, TagBFP
Backbone Map
pKVL2-U6gRNA_SAM(BbsI)-PGKpuroBFP-W vector formula
Click to view the full image
CRISPR iScreen™ Product Strength
  • 35+ Libraries
    100+ Cas9 cell lines for screening
    35+ Library types in stock, fulfilling different research needs Cas9 cells with high activity, good cell condition, easily accelerate CRISPR library construction.
    1
  • Plasmid
    Coverage>99%, uniformity<10
    The use of self-developed library specific competent cell makes it easier to capture exogenous DNA, with high transformation efficiency and low mutation risk.
    2
  • Cell Pool
    Coverage rate up to 99%
    Exclusive cell pool preparation process can achieve large-scale and standardized production of library cell pool, achieving fewer differences between batches and high repeatability.
    3
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Cas9 Stable Cell Line
The Cas9 cell lines in our cell bank can stably express Cas9 protein. So gene knockout can be achieved by transfecting gRNA. Simultaneously transfecting gRNA and donor DNA can achieve gene knock-in/point mutation, effectively improving experimental efficiency.
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