Detection of Marker Protein Expression

Location: Home > Gene Editing Services > Cell Assay >

< >
Detection of Marker Protein Expression
Immunofluorescence technique, also known as Fluorescent Antibody Technique, is one of the earliest development of labeled immune technology. It is a technology based on immunology, biochemistry and microscopy. For a long time, some scholars have tried to combine antibody molecules with some tracer substances, and use antigen antibody reaction to locate antigen substances in tissues or cells.

Technical details
Immunofluorescence technique is based on the principle of antigen-antibody reaction. Firstly, the known antigen or antibody is labeled with fluorescent groups, and then the fluorescent antibody (or antigen) is used as a probe to detect the corresponding antigen (or antibody) in cells or tissues. The nature and location of the antibody can be seen by fluorescence microscope.



Case study


Reference
Im, Kyuseok et al. “An Introduction to Performing Immunofluorescence Staining.” Methods in molecular biology (Clifton, N.J.) vol. 1897 (2019): 299-311. doi:10.1007/978-1-4939-8935-5_26


Service turnaround and deliverables

Service turnaround

5-10 business days, the actual turnaround depends on cell growth

Customer provides

cell line, antibody, substrate, substrate treatment method, and experiment design

Deliverables

raw data, analytical result, project report.