Knockout Cell lines | study of non-coding RNA

Gene Editing Services

Ubigene has modified over 5000 genes from more than 200 cell lines with our exclusive innovation CRISPR-U™ technology. At the same time, we already provide customers with high quality gene-editing tools for cell or animal research worldwide.

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EZ-editor™
Gene-editing Series Products

With 14 years of experience, Ubigene has exclusively innovated and developed 6 product lines, fullfilling all kinds of needs from researchers. Experiment process simplified, efficiency improved, achieving our aim of 'Make genome editing easier'!

Red Cotton™ gRNA Plasmid Bank

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EZ-editor™ Culture Medium

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Knockout-cell-lysate

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EZ-editor™ Cell Line

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EGFP Stable Cell Lines

EZ-editor™ Kits

Monoclone Genotype Validation Kit (extraction free)

EZ-editor™ Gene Knockout kit

Mouse Genotyping Kit

Lentiviral Packaging Kit

Lentivirus

IVD positive controls

Point Mutation gDNA/Cell Line

Red Cotton Gene knockout Project

Ubigene exclusive KO Cell Line Bank, over 5000 KO cell lines, covering thousands of genes from 8 popular signaling pathways and nearly 100 diseases.

Red Cotton CRISPR Gene Editing Designer

Gene Editing Cell Designer

Gene Editing Point Mutation Designer

Gene Editing Microbe Designer

Gene Editing Tool Kit

Red Cotton Gene knockout Project

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With the progress of studying microRNA, it is revealed that ncRNA plays an important role in post-transcriptional regulation, cell growth, differentiation and proliferation. MicroRNA, circRNA and lncRNA are the most commonly studied ncRNA. Since 2018, over 60,000 publications are related to non-coding RNA.

What are the methods to study the function of non-coding RNA? And how to choose the best method for your research?

Overexpression, RNA mimics, and RNA agonists can be used for functional acquisition verification. For the study of functional deficiency, RNAi, RNA inhibitors, antagonists, antisense oligonucleotides (ASO), promoter knockout, locus knockout are commonly used.

RNAi cannot silence non-coding RNA like some circRNA and lncRNA. RNAi plays a role in the post-transcriptional level, that is to say, RNA must be located in the cytoplasm to be interfered. But lncRNA and circRNA is selectively distributed in the nucleus and cytoplasm.
RNA inhibitors and antagonists are mainly used in the research of microRNA, but not in the research of circRNA and lncRNA.
ASO cannot completely prevent RNA transcription and interfere with expression at the DNA level. In addition, using ASO method cannot generate stable transgenic cell lines.

Locus knockout and promoter knockout are the most effective methods to study the function of long non-coding RNA.

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