HEK293-CAS9 cell line

×

Subscribe Us

By subscription, you consent to allow Ubigene Biosciences to store and process the information provided above to deliver the latest news, research spotlight, and promotions. You can unsubscribe from these communications at anytime.
location: Home > Products > Cas9 Stable Cell Lines > HEK293-CAS9
Cas9 Stable Cell Lines
  • All products
  • Red Cotton™ gRNA Plasmid Bank
  • KO Cell Lines
  • Wild-type Cell Lines
  • Cas9 Stable Cell Lines
  • Luciferase Stable Cell Lines
  • EGFP Stable Cell Lines
  • Stem Cell Lines
  • Transfection Culture Medium
  • Cell Monoclonal Culture Medium
  • EZ-Stem™ Cell Culture Medium
  • CRISPR-iScreen™ Library Plasmid
  • CRISPR-iScreen™ Library Virus
  • CRISPR-iScreen™ Cell Pool
  • EZ-cryo™ cell freezing medium
  • Kits
  • Lentivirus
  • HEK293 HEK293-CAS9-HEK293
  • HEK293-CAS9 cell line HEK293-CAS9-产品图
HEK293-CAS9 Copy Product Info
Catalog#: YC-A008-Cas9-H
Size: 1*10^6
Culture method: 90%DMEM+10% FBS
  • HEK293 HEK293-CAS9-HEK293
  • HEK293-CAS9 cell line HEK293-CAS9-产品图
HEK293-CAS9 Copy Product Info
Catalog#: YC-A008-Cas9-H
Size: 1*10^6
Culture method: 90%DMEM+10% FBS

Detailed Product Information

Cell Info

Product category Cas9 Stable Cell Lines Mycoplasma test negative
Cell Morphology Epithelial-like, adherent Culture method 90%DMEM+10% FBS
Passage ratio 1:3~1:6 Species Human
Expression gene Cas9 Resistance gene hygro
Product category
Cas9 Stable Cell Lines
Mycoplasma test
negative
Cell Morphology
Epithelial-like, adherent
Culture method
90%DMEM+10% FBS
Passage ratio
1:3~1:6
Species
Human
Expression gene
Cas9
Resistance gene
hygro
Recombinant Cas9 protein derived from Streptococcus pyogenes is a protein that plays a key role in CRISPR/Cas9 system. It has endonuclease activity; Under the guidance of sgRNA, Cas9 protein can move to the target gene site, cut DNA accurately, and cause DNA double-strand breaks. Ubigene's HEK293 cell line was constructed on the basis of wild-type HEK293 cells by lentiviral transduction of the engineered Cas9 protein gene, which can stably express Cas9 protein for a long time. Using this cell, efficient gene knockout, knock-in and point mutation in HEK293 cells can be achieved by only transfecting sgRNA or co-transfecting sgRNA and donor DNA. It can also be used for the construction of cell knockout library and easily solve the problem of Cas9 transfection difficulty.

Product Validation Data

RT-qPCR
Sample Name Target Name Cт Mean ΔCт
HEK293-CAS9 Cas9 16.85865784 -1.60838890
HEK293-CAS9 β-actin 18.46704674
HEK293 Cas9 34.38785934 18.06243705
HEK293 β-actin 16.32542229
Cutting Efficiency Validation
HEK293-CAS9 cell line Product Validation Data(RT-QPCR)
Note: The above figure shows the sequencing peaks of the HEK293-CAS9 stable cell pool which is electroporated by DCAF11 gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:HEK293
STR Info (Cell bank)
Cell Line:HEK-293.2sus
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 8888
D13S317 12141214
D7S820 11121112
D16S539 913913
VWA 16191619
TH01 79.379.3
AMEL XXXX
TPOX 11111111
CSF1PO 12121212
D12S391 1921
FGA 2323
D2S1338 1919
D21S11 2830.2
D18S51 1718
D8S1179 1214
D3S1358 1517
D6S1043 1111
PENTAE 715
D19S433 1818
PENTAD 910
D1S1656 1517.3
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

  • icon
    Easy to use
    Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
  • icon
    High-efficient KO
    These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
  • icon
    Curated cells
    Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.

Related Popular Products

KO Cell Lines

  • IQGAP2 Knockout cell line (HEK293)

    Organism:
    Gene Name: IQGAP2
    Gene ID: 10788
    Size: 1*10^6
    Catalog#: YKO-H1478
    YKO-H1478(HEK293)
  • IRF3 Knockout cell line (HEK293)

    Organism:
    Gene Name: IRF3
    Gene ID: 3661
    Size: 1*10^6
    Catalog#: YKO-H1475
    YKO-H1475(HEK293)
  • NCOA4 Knockout cell line (HEK293)

    Organism:
    Gene Name: NCOA4
    Gene ID: 8031
    Size: 1*10^6
    Catalog#: YKO-H1473
    YKO-H1473(HEK293)

Related Popular Products

KO Cell Lines

  • IQGAP2 Knockout cell line (HEK293)

    Organism:
    Gene Name: IQGAP2
    Gene ID: 10788
    Size: 1*10^6
    Catalog#: YKO-H1478
  • IRF3 Knockout cell line (HEK293)

    Organism:
    Gene Name: IRF3
    Gene ID: 3661
    Size: 1*10^6
    Catalog#: YKO-H1475
  • NCOA4 Knockout cell line (HEK293)

    Organism:
    Gene Name: NCOA4
    Gene ID: 8031
    Size: 1*10^6
    Catalog#: YKO-H1473

×

Search documentation

Literature:

Name: *

Company: *

Telephone: *

Email:

Notes: After submitting the order, we will contact you as soon as possible.

request now
Contact us