HGC-27-CAS9 cell line

×

Subscribe Us

By subscription, you consent to allow Ubigene Biosciences to store and process the information provided above to deliver the latest news, research spotlight, and promotions. You can unsubscribe from these communications at anytime.
location: Home > Products > Cas9 Stable Cell Lines > HGC-27-CAS9
Cas9 Stable Cell Lines
  • All products
  • Red Cotton™ gRNA Plasmid Bank
  • KO Cell Lines
  • Wild-type Cell Lines
  • Cas9 Stable Cell Lines
  • Luciferase Stable Cell Lines
  • EGFP Stable Cell Lines
  • Stem Cell Lines
  • Transfection Culture Medium
  • Cell Monoclonal Culture Medium
  • EZ-Stem™ Cell Culture Medium
  • CRISPR-iScreen™ Library Plasmid
  • CRISPR-iScreen™ Library Virus
  • CRISPR-iScreen™ Cell Pool
  • EZ-cryo™ cell freezing medium
  • Kits
  • Lentivirus
  • Wild-type Cell Lines-1 HGC-27-CAS9-Wild-type Cell Lines-1
  • Wild-type Cell Lines-2 HGC-27-CAS9-Wild-type Cell Lines-2
  • Transfection HGC-27-CAS9-Transfection
  • Monoclonal HGC-27-CAS9-Monoclonal
  • HGC-27-CAS9 cell line HGC-27-CAS9-产品图
HGC-27-CAS9 Copy Product Info
Catalog#: YC-C079-Cas9-H
Size: 1*10^6
Culture method: 90%RPMI-1640+10% FBS
  • Wild-type Cell Lines-1 HGC-27-CAS9-Wild-type Cell Lines-1
  • Wild-type Cell Lines-2 HGC-27-CAS9-Wild-type Cell Lines-2
  • Transfection HGC-27-CAS9-Transfection
  • Monoclonal HGC-27-CAS9-Monoclonal
  • HGC-27-CAS9 cell line HGC-27-CAS9-产品图
HGC-27-CAS9 Copy Product Info
Catalog#: YC-C079-Cas9-H
Size: 1*10^6
Culture method: 90%RPMI-1640+10% FBS

Detailed Product Information

Cell Info

Product category Cas9 Stable Cell Lines Mycoplasma test negative
Cell Morphology Endothelial cells, adherent Culture method 90%RPMI-1640+10% FBS
Passage ratio 1:3~1:4 Species Human
Expression gene Cas9 Resistance gene hygro
Product category
Cas9 Stable Cell Lines
Mycoplasma test
negative
Cell Morphology
Endothelial cells, adherent
Culture method
90%RPMI-1640+10% FBS
Passage ratio
1:3~1:4
Species
Human
Expression gene
Cas9
Resistance gene
hygro
Recombinant Cas9 protein derived from Streptococcus pyogenes is a protein that plays a key role in CRISPR/Cas9 system. It has endonuclease activity; Under the guidance of sgRNA, Cas9 protein can move to the target gene site, cut DNA accurately, and cause DNA double-strand breaks. Ubigene's HGC-27 cell line was constructed on the basis of wild-type HGC-27 cells by lentiviral transduction of the engineered Cas9 protein gene, which can stably express Cas9 protein for a long time. Using this cell, efficient gene knockout, knock-in and point mutation in HGC-27 cells can be achieved by only transfecting sgRNA or co-transfecting sgRNA and donor DNA. It can also be used for the construction of cell knockout library and easily solve the problem of Cas9 transfection difficulty.

Product Validation Data

RT-qPCR
Sample Name Target Name Cт Mean ΔCт
HGC-27-CAS9 Cas9 19.70162010 5.17640495
HGC-27-CAS9 β-actin 14.52521515
HGC-27 Cas9 33.80643082 19.62654114
HGC-27 β-actin 14.17988968
Cutting Efficiency Validation
HGC-27-CAS9 cell line Product Validation Data(RT-QPCR)
Note: The above figure shows the sequencing peaks of the HGC-27-CAS9 stable cell pool which is electroporated by PIN1 gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:HGC-27
STR Info (Cell bank)
Cell Line:HGC-27
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12121212
D13S317 10111011
D7S820 111213.0111213.0
D16S539 10111011
VWA 14141414
TH01 9999
AMEL XXXX
TPOX 8888
CSF1PO 12121212
D12S391 2021
FGA 2222
D2S1338 2224
D21S11 303334.0
D18S51 1617
D8S1179 1111
D3S1358 1717
D6S1043 1818
PENTAE 1818
D19S433 1414
PENTAD 913
D1S1656 1416
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

  • icon
    Easy to use
    Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
  • icon
    High-efficient KO
    These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
  • icon
    Curated cells
    Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.

Related Popular Products

KO Cell Lines

  • PIN1 Knockout cell line (HGC-27)

    Organism: Human
    Gene Name: PIN1
    Gene ID: 5300
    Size: 1*10^6
    Catalog#: YKO-H864
    YKO-H864(HGC-27)
  • FGFR1 Knockout cell line(HGC-27)

    Organism: Human
    Gene Name: FGFR1
    Gene ID: 2260
    Size: 1*10^6
    Catalog#: YKO-H209
    YKO-H209(HGC-27)

Luciferase Stable Cell Lines

Wild-type Cell Lines

Related Popular Products

KO Cell Lines

  • PIN1 Knockout cell line (HGC-27)

    Organism: Human
    Gene Name: PIN1
    Gene ID: 5300
    Size: 1*10^6
    Catalog#: YKO-H864
  • FGFR1 Knockout cell line(HGC-27)

    Organism: Human
    Gene Name: FGFR1
    Gene ID: 2260
    Size: 1*10^6
    Catalog#: YKO-H209

Luciferase Stable Cell Lines

Wild-type Cell Lines


×

Search documentation

Literature:

Name: *

Company: *

Telephone: *

Email:

Notes: After submitting the order, we will contact you as soon as possible.

request now
Contact us