Hela-CAS9 cell line

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location： Home > Products > Cas9 Stable Cell Lines > Hela-CAS9

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Hela-CAS9 Order

Hela-CAS9 cell line
Catalog#
YC-A012-Cas9-H
Size
1*10^6
Transfection Culture Medium
Transfection Culture medium-A
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Datasheet
Hela-CAS9 cell line
Order

Detailed Product Information

Product category
Cas9 Stable Cell Lines
cell name
Hela-CAS9
Morphology
Epithelial-like, adherent
Passage ratio
1：2-1：4
Species
Human
Expression gene
Cas9
Resistance gene
hygro
Construction method
Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10% FBS
Description
Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the Hela cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a Hela-CAS9 cell line that can maintain long-term, stable and high expression.
Product Validation Data（RT-QPCR）
Sample Name Target Name Cт Mean ΔCт
HeLa-CAS9 Cas9 14.15423965 -0.64070892
HeLa-CAS9 h-actin-B 14.79494858
Hela Cas9 30.20011902 15.75909424
Hela h-actin-B 14.44102478
Cutting Efficiency Validation
Hela-CAS9 cell line Product Validation Data（RT-QPCR）
Note: The above figure shows the sequencing peaks of the HeLa-CAS9 stable cell pool which is electroporated by NONO gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:Hela		 STR Info (Cell bank)
Cell Line:HELA
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 1213.31213.3
D7S820 812812
D16S539 910910
VWA 16181618
TH01 7777
AMEL XXXX
TPOX 812812
CSF1PO 910910
D12S391 202425.0
FGA 2121
D2S1338 1717
D21S11 2728
D18S51 1616
D8S1179 1213
D3S1358 1518
D6S1043 1819
PENTAE 717
D19S433 1314
PENTAD 815
D1S1656 1215
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

  • Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
    01
  • High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
    02
  • High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
    03
Hela-CAS9 cell line
Hela-CAS9
Catalog#
YC-A012-Cas9-H
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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