ABCA1 Knockout cell line (Hep G2)

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ABCA1 Knockout cell line (Hep G2) Order

ABCA1 Knockout cell line (Hep G2)
Catalog#
YKO-HT1453
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
ABCA1 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
ABCA1 Knockout cell line (Hep G2)
Species
Human
Gene Name
ABCA1
Gene ID
19
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 4 (based on transcript ABCA1-202)
Validation: PCR+Sanger Sequencing
ABCA1 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ABCA1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ABCA1 gene knockout ABCA1 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
ABCA1 Knockout cell line (Hep G2)
ABCA1 Knockout cell line (Hep G2)
Catalog#
YKO-HT1453
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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Gene Knockout kit

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  • ABCA1 Knockout cell line (HeLa)

    ABCA1 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: ABCA1
    Gene ID: 19
    Size: 1*10^6
    Catalog#: YKO-XN85015
    YKO-XN85015(HeLa)
  • ABCA1 Knockout cell line (A549)

    ABCA1 Knockout cell line (A549)
      inquiry   More details

    Organism: Human
    Gene Name: ABCA1
    Gene ID: 19
    Size: 1*10^6
    Catalog#: YKO-XN85013
    YKO-XN85013(A549)
  • ABCA1 Knockout cell line (U-87 MG)

    ABCA1 Knockout cell line (U-87 MG)
      inquiry   More details

    Organism: Human
    Gene Name: ABCA1
    Gene ID: 19
    Size: 1*10^6
    Catalog#: YKO-H247
    YKO-H247(U-87 MG)

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EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • ABCA1 Knockout cell line (HeLa)

    ABCA1 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: ABCA1
    Gene ID: 19
    Size: 1*10^6
    Catalog#: YKO-XN85015
      inquiry   More details
  • ABCA1 Knockout cell line (A549)

    ABCA1 Knockout cell line (A549)

    Organism: Human
    Gene Name: ABCA1
    Gene ID: 19
    Size: 1*10^6
    Catalog#: YKO-XN85013
      inquiry   More details
  • ABCA1 Knockout cell line (U-87 MG)

    ABCA1 Knockout cell line (U-87 MG)

    Organism: Human
    Gene Name: ABCA1
    Gene ID: 19
    Size: 1*10^6
    Catalog#: YKO-H247
      inquiry   More details

ABCA1 Knockout cell line (Hep G2) Order

ABCA1 Knockout cell line (Hep G2)
Catalog#
YKO-HT1453
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
ABCA1 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
ABCA1 Knockout cell line (Hep G2)
Species
Human
Gene Name
ABCA1
Gene ID
19
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 4 (based on transcript ABCA1-202)
Validation: PCR+Sanger Sequencing
ABCA1 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ABCA1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ABCA1 gene knockout ABCA1 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
ABCA1 Knockout cell line (Hep G2)
ABCA1 Knockout cell line (Hep G2)
Catalog#
YKO-HT1453
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • ABCA1 Knockout cell line (HeLa)

    ABCA1 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: ABCA1
    Gene ID: 19
    Size: 1*10^6
    Catalog#: YKO-XN85015
    YKO-XN85015(HeLa)
  • ABCA1 Knockout cell line (A549)

    ABCA1 Knockout cell line (A549)
      inquiry   More details

    Organism: Human
    Gene Name: ABCA1
    Gene ID: 19
    Size: 1*10^6
    Catalog#: YKO-XN85013
    YKO-XN85013(A549)
  • ABCA1 Knockout cell line (U-87 MG)

    ABCA1 Knockout cell line (U-87 MG)
      inquiry   More details

    Organism: Human
    Gene Name: ABCA1
    Gene ID: 19
    Size: 1*10^6
    Catalog#: YKO-H247
    YKO-H247(U-87 MG)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • ABCA1 Knockout cell line (HeLa)

    ABCA1 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: ABCA1
    Gene ID: 19
    Size: 1*10^6
    Catalog#: YKO-XN85015
      inquiry   More details
  • ABCA1 Knockout cell line (A549)

    ABCA1 Knockout cell line (A549)

    Organism: Human
    Gene Name: ABCA1
    Gene ID: 19
    Size: 1*10^6
    Catalog#: YKO-XN85013
      inquiry   More details
  • ABCA1 Knockout cell line (U-87 MG)

    ABCA1 Knockout cell line (U-87 MG)

    Organism: Human
    Gene Name: ABCA1
    Gene ID: 19
    Size: 1*10^6
    Catalog#: YKO-H247
      inquiry   More details

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