Cd47 Knockout cell line (Raw 264.7)

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Cd47 Knockout cell line (Raw 264.7) Order

Cd47 Knockout cell line (Raw 264.7)
Catalog#
YKO-MT0128
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Cd47 Knockout cell line (Raw 264.7)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
Cd47 Knockout cell line (Raw 264.7)
Species
Mouse
Gene Name
Cd47
Gene ID
16423
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript Cd47-208)
Validation: PCR+Sanger Sequencing

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Cd47 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Raw 264.7 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Cd47 gene knockout Cd47 Knockout cell line (Raw 264.7) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
Cd47 Knockout cell line (Raw 264.7)
Cd47 Knockout cell line (Raw 264.7)
Catalog#
YKO-MT0128
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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