Cybb Knockout cell line (Raw 264.7)

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location： Home > Products > KO Cell Lines > Cybb Knockout cell line (Raw 264.7)

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Cybb Knockout cell line (Raw 264.7) Order

Cybb Knockout cell line (Raw 264.7)
Catalog#
YKO-MT0081
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Cybb Knockout cell line (Raw 264.7)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
Cybb Knockout cell line (Raw 264.7)
Species
Mouse
Gene Name
Cybb
Gene ID
13058
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 3 (based on transcript Cybb-201)
Validation: PCR+Sanger Sequencing
Cybb Knockout cell line (Raw 264.7) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Cybb gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Raw 264.7 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Cybb gene knockout Cybb Knockout cell line (Raw 264.7) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
Cybb Knockout cell line (Raw 264.7)
Cybb Knockout cell line (Raw 264.7)
Catalog#
YKO-MT0081
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

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  • CYBB Knockout cell line (HCT 116)

    CYBB Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: CYBB
    Gene ID: 1536
    Size: 1*10^6
    Catalog#: YKO-HS6681
      inquiry   More details
  • CYBB Knockout cell line (HEK293)

    CYBB Knockout cell line (HEK293)

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    Gene Name: CYBB
    Gene ID: 1536
    Size: 1*10^6
    Catalog#: YKO-H462
      inquiry   More details

Cybb Knockout cell line (Raw 264.7) Order

Cybb Knockout cell line (Raw 264.7)
Catalog#
YKO-MT0081
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Cybb Knockout cell line (Raw 264.7)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
Cybb Knockout cell line (Raw 264.7)
Species
Mouse
Gene Name
Cybb
Gene ID
13058
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 3 (based on transcript Cybb-201)
Validation: PCR+Sanger Sequencing
Cybb Knockout cell line (Raw 264.7) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Cybb gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Raw 264.7 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Cybb gene knockout Cybb Knockout cell line (Raw 264.7) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
Cybb Knockout cell line (Raw 264.7)
Cybb Knockout cell line (Raw 264.7)
Catalog#
YKO-MT0081
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

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  • CYBB Knockout cell line (HCT 116)

    CYBB Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: CYBB
    Gene ID: 1536
    Size: 1*10^6
    Catalog#: YKO-HS6681
    YKO-HS6681(HCT 116)
  • CYBB Knockout cell line (HEK293)

    CYBB Knockout cell line (HEK293)
      inquiry   More details

    Organism: Human
    Gene Name: CYBB
    Gene ID: 1536
    Size: 1*10^6
    Catalog#: YKO-H462
    YKO-H462(HEK293)

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Wild-type Cell Lines

  • RAW 264.7 cell line

    Mouse Macrophages transformed by leukemia virus(RAW 264.7)

    Price (USD): -
    Catalog#: YC-C020
    Size: 1*10^6
    Instruction: Mouse Macrophages transformed by leukemia virus(RAW 264.7)
      inquiry   More details

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • CYBB Knockout cell line (HCT 116)

    CYBB Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: CYBB
    Gene ID: 1536
    Size: 1*10^6
    Catalog#: YKO-HS6681
      inquiry   More details
  • CYBB Knockout cell line (HEK293)

    CYBB Knockout cell line (HEK293)

    Organism: Human
    Gene Name: CYBB
    Gene ID: 1536
    Size: 1*10^6
    Catalog#: YKO-H462
      inquiry   More details

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