FMR1 Knockout cell line (SH-SY5Y)

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location： Home > Products > KO Cell Lines > FMR1 Knockout cell line (SH-SY5Y)

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FMR1 Knockout cell line (SH-SY5Y) Order

FMR1 Knockout cell line (SH-SY5Y)
Catalog#
YKO-H1149
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
FMR1 Knockout cell line (SH-SY5Y)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
FMR1 Knockout cell line (SH-SY5Y)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:2-1:3
Species
Human
Gene Name
FMR1
Gene ID
2332
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10% FBS
Target region
Knockout region: Exon 7 (based on transcript FMR1-205)
Validation: PCR+Sanger Sequencing
FMR1 Knockout cell line (SH-SY5Y) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:SH-SY5Y		 STR Info (Cell bank)
Cell Line:SH-SY5Y
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12121212
D13S317 11111111
D7S820 710710
D16S539 813813
VWA 14181418
TH01 710710
AMEL XXXX
TPOX 811811
CSF1PO 11111111
D12S391 1822
FGA 23.224
D2S1338 1719
D21S11 3131.2
D18S51 1316
D8S1179 1515
D3S1358 1516
D6S1043 1218
PENTAE 711
D19S433 1314
PENTAD 1012
D1S1656 1212
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the FMR1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the SH-SY5Y cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, FMR1 gene knockout FMR1 Knockout cell line (SH-SY5Y) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
FMR1 Knockout cell line (SH-SY5Y)
FMR1 Knockout cell line (SH-SY5Y)
Catalog#
YKO-H1149
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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