FURIN Knockout cell line (Hep G2)

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FURIN Knockout cell line (Hep G2) Order

FURIN Knockout cell line (Hep G2)
Catalog#
YKO-HT1737
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
FURIN Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
FURIN Knockout cell line (Hep G2)
Species
Human
Gene Name
FURIN
Gene ID
5045
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript FURIN-201)
Validation: PCR+Sanger Sequencing
FURIN Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the FURIN gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, FURIN gene knockout FURIN Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
FURIN Knockout cell line (Hep G2)
FURIN Knockout cell line (Hep G2)
Catalog#
YKO-HT1737
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • FURIN Knockout cell line (THP-1)

    FURIN Knockout cell line (THP-1)
      inquiry   More details

    Organism: Human
    Gene Name: FURIN
    Gene ID: 5045
    Size: 1*10^6
    Catalog#: YKO-HT0320
    YKO-HT0320(THP-1)
  • FURIN Knockout cell line (HCT 116)

    FURIN Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: FURIN
    Gene ID: 5045
    Size: 1*10^6
    Catalog#: YKO-XN88621
    YKO-XN88621(HCT 116)
  • FURIN Knockout cell line (HEK293)

    FURIN Knockout cell line (HEK293)
      inquiry   More details

    Organism: Human
    Gene Name: FURIN
    Gene ID: 5045
    Size: 1*10^6
    Catalog#: YKO-XN88620
    YKO-XN88620(HEK293)

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Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • FURIN Knockout cell line (THP-1)

    FURIN Knockout cell line (THP-1)

    Organism: Human
    Gene Name: FURIN
    Gene ID: 5045
    Size: 1*10^6
    Catalog#: YKO-HT0320
      inquiry   More details
  • FURIN Knockout cell line (HCT 116)

    FURIN Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: FURIN
    Gene ID: 5045
    Size: 1*10^6
    Catalog#: YKO-XN88621
      inquiry   More details
  • FURIN Knockout cell line (HEK293)

    FURIN Knockout cell line (HEK293)

    Organism: Human
    Gene Name: FURIN
    Gene ID: 5045
    Size: 1*10^6
    Catalog#: YKO-XN88620
      inquiry   More details

FURIN Knockout cell line (Hep G2) Order

FURIN Knockout cell line (Hep G2)
Catalog#
YKO-HT1737
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
FURIN Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
FURIN Knockout cell line (Hep G2)
Species
Human
Gene Name
FURIN
Gene ID
5045
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript FURIN-201)
Validation: PCR+Sanger Sequencing
FURIN Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the FURIN gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, FURIN gene knockout FURIN Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
FURIN Knockout cell line (Hep G2)
FURIN Knockout cell line (Hep G2)
Catalog#
YKO-HT1737
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • FURIN Knockout cell line (THP-1)

    FURIN Knockout cell line (THP-1)
      inquiry   More details

    Organism: Human
    Gene Name: FURIN
    Gene ID: 5045
    Size: 1*10^6
    Catalog#: YKO-HT0320
    YKO-HT0320(THP-1)
  • FURIN Knockout cell line (HCT 116)

    FURIN Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: FURIN
    Gene ID: 5045
    Size: 1*10^6
    Catalog#: YKO-XN88621
    YKO-XN88621(HCT 116)
  • FURIN Knockout cell line (HEK293)

    FURIN Knockout cell line (HEK293)
      inquiry   More details

    Organism: Human
    Gene Name: FURIN
    Gene ID: 5045
    Size: 1*10^6
    Catalog#: YKO-XN88620
    YKO-XN88620(HEK293)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • FURIN Knockout cell line (THP-1)

    FURIN Knockout cell line (THP-1)

    Organism: Human
    Gene Name: FURIN
    Gene ID: 5045
    Size: 1*10^6
    Catalog#: YKO-HT0320
      inquiry   More details
  • FURIN Knockout cell line (HCT 116)

    FURIN Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: FURIN
    Gene ID: 5045
    Size: 1*10^6
    Catalog#: YKO-XN88621
      inquiry   More details
  • FURIN Knockout cell line (HEK293)

    FURIN Knockout cell line (HEK293)

    Organism: Human
    Gene Name: FURIN
    Gene ID: 5045
    Size: 1*10^6
    Catalog#: YKO-XN88620
      inquiry   More details

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