GORASP2 Knockout cell line (Hep G2)

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GORASP2 Knockout cell line (Hep G2) Order

GORASP2 Knockout cell line (Hep G2)
Catalog#
YKO-HT3743
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
GORASP2 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
GORASP2 Knockout cell line (Hep G2)
Species
Human
Gene Name
GORASP2
Gene ID
26003
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 3 (based on transcript GORASP2-201)
Validation: PCR+Sanger Sequencing
GORASP2 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the GORASP2 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, GORASP2 gene knockout GORASP2 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
GORASP2 Knockout cell line (Hep G2)
GORASP2 Knockout cell line (Hep G2)
Catalog#
YKO-HT3743
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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Gene Knockout kit

EZ-cryo™ cell freezing medium

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  • GORASP2 Knockout cell line(HEK293)

    GORASP2 Knockout cell line(HEK293)

    Organism: Human
    Gene Name: GORASP2
    Gene ID: 26003
    Size: 1*10^6
    Catalog#: YKO-H585
    YKO-H585(HEK293)
  • GORASP2 Knockout cell line (HeLa)

    GORASP2 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: GORASP2
    Gene ID: 26003
    Size: 1*10^6
    Catalog#: YKO-XN51400
    YKO-XN51400(HeLa)
  • GORASP2 Knockout cell line (HCT 116)

    GORASP2 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: GORASP2
    Gene ID: 26003
    Size: 1*10^6
    Catalog#: YKO-XN51399
    YKO-XN51399(HCT 116)

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Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium

KO Cell Lines

  • GORASP2 Knockout cell line(HEK293)

    GORASP2 Knockout cell line(HEK293)

    Organism: Human
    Gene Name: GORASP2
    Gene ID: 26003
    Size: 1*10^6
    Catalog#: YKO-H585
  • GORASP2 Knockout cell line (HeLa)

    GORASP2 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: GORASP2
    Gene ID: 26003
    Size: 1*10^6
    Catalog#: YKO-XN51400
  • GORASP2 Knockout cell line (HCT 116)

    GORASP2 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: GORASP2
    Gene ID: 26003
    Size: 1*10^6
    Catalog#: YKO-XN51399

GORASP2 Knockout cell line (Hep G2) Order

GORASP2 Knockout cell line (Hep G2)
Catalog#
YKO-HT3743
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
GORASP2 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
GORASP2 Knockout cell line (Hep G2)
Species
Human
Gene Name
GORASP2
Gene ID
26003
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 3 (based on transcript GORASP2-201)
Validation: PCR+Sanger Sequencing
GORASP2 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the GORASP2 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, GORASP2 gene knockout GORASP2 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
GORASP2 Knockout cell line (Hep G2)
GORASP2 Knockout cell line (Hep G2)
Catalog#
YKO-HT3743
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • GORASP2 Knockout cell line(HEK293)

    GORASP2 Knockout cell line(HEK293)

    Organism: Human
    Gene Name: GORASP2
    Gene ID: 26003
    Size: 1*10^6
    Catalog#: YKO-H585
    YKO-H585(HEK293)
  • GORASP2 Knockout cell line (HeLa)

    GORASP2 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: GORASP2
    Gene ID: 26003
    Size: 1*10^6
    Catalog#: YKO-XN51400
    YKO-XN51400(HeLa)
  • GORASP2 Knockout cell line (HCT 116)

    GORASP2 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: GORASP2
    Gene ID: 26003
    Size: 1*10^6
    Catalog#: YKO-XN51399
    YKO-XN51399(HCT 116)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium

KO Cell Lines

  • GORASP2 Knockout cell line(HEK293)

    GORASP2 Knockout cell line(HEK293)

    Organism: Human
    Gene Name: GORASP2
    Gene ID: 26003
    Size: 1*10^6
    Catalog#: YKO-H585
  • GORASP2 Knockout cell line (HeLa)

    GORASP2 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: GORASP2
    Gene ID: 26003
    Size: 1*10^6
    Catalog#: YKO-XN51400
  • GORASP2 Knockout cell line (HCT 116)

    GORASP2 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: GORASP2
    Gene ID: 26003
    Size: 1*10^6
    Catalog#: YKO-XN51399

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