GSTP1 Knockout cell line (THP-1)

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GSTP1 Knockout cell line (THP-1) Order

GSTP1 Knockout cell line (THP-1)
Catalog#
YKO-HT0172
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
GSTP1 Knockout cell line (THP-1)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
GSTP1 Knockout cell line (THP-1)
Species
Human
Gene Name
GSTP1
Gene ID
2950
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 3 (based on transcript GSTP1-202)
Validation: PCR+Sanger Sequencing
GSTP1 Knockout cell line (THP-1) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the GSTP1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the THP-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, GSTP1 gene knockout GSTP1 Knockout cell line (THP-1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
GSTP1 Knockout cell line (THP-1)
GSTP1 Knockout cell line (THP-1)
Catalog#
YKO-HT0172
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

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Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • GSTP1 Knockout cell line (HeLa)

    GSTP1 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: GSTP1
    Gene ID: 2950
    Size: 1*10^6
    Catalog#: YKO-HS4402
    YKO-HS4402(HeLa)
  • GSTP1 Knockout cell line (HCT 116)

    GSTP1 Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: GSTP1
    Gene ID: 2950
    Size: 1*10^6
    Catalog#: YKO-HS3279
    YKO-HS3279(HCT 116)
  • GSTP1 Knockout cell line (HEK293)

    GSTP1 Knockout cell line (HEK293)
      inquiry   More details

    Organism: Human
    Gene Name: GSTP1
    Gene ID: 2950
    Size: 1*10^6
    Catalog#: YKO-HS2154
    YKO-HS2154(HEK293)

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Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • GSTP1 Knockout cell line (HeLa)

    GSTP1 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: GSTP1
    Gene ID: 2950
    Size: 1*10^6
    Catalog#: YKO-HS4402
      inquiry   More details
  • GSTP1 Knockout cell line (HCT 116)

    GSTP1 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: GSTP1
    Gene ID: 2950
    Size: 1*10^6
    Catalog#: YKO-HS3279
      inquiry   More details
  • GSTP1 Knockout cell line (HEK293)

    GSTP1 Knockout cell line (HEK293)

    Organism: Human
    Gene Name: GSTP1
    Gene ID: 2950
    Size: 1*10^6
    Catalog#: YKO-HS2154
      inquiry   More details

GSTP1 Knockout cell line (THP-1) Order

GSTP1 Knockout cell line (THP-1)
Catalog#
YKO-HT0172
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
GSTP1 Knockout cell line (THP-1)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
GSTP1 Knockout cell line (THP-1)
Species
Human
Gene Name
GSTP1
Gene ID
2950
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 3 (based on transcript GSTP1-202)
Validation: PCR+Sanger Sequencing
GSTP1 Knockout cell line (THP-1) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the GSTP1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the THP-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, GSTP1 gene knockout GSTP1 Knockout cell line (THP-1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
GSTP1 Knockout cell line (THP-1)
GSTP1 Knockout cell line (THP-1)
Catalog#
YKO-HT0172
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • GSTP1 Knockout cell line (HeLa)

    GSTP1 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: GSTP1
    Gene ID: 2950
    Size: 1*10^6
    Catalog#: YKO-HS4402
    YKO-HS4402(HeLa)
  • GSTP1 Knockout cell line (HCT 116)

    GSTP1 Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: GSTP1
    Gene ID: 2950
    Size: 1*10^6
    Catalog#: YKO-HS3279
    YKO-HS3279(HCT 116)
  • GSTP1 Knockout cell line (HEK293)

    GSTP1 Knockout cell line (HEK293)
      inquiry   More details

    Organism: Human
    Gene Name: GSTP1
    Gene ID: 2950
    Size: 1*10^6
    Catalog#: YKO-HS2154
    YKO-HS2154(HEK293)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • GSTP1 Knockout cell line (HeLa)

    GSTP1 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: GSTP1
    Gene ID: 2950
    Size: 1*10^6
    Catalog#: YKO-HS4402
      inquiry   More details
  • GSTP1 Knockout cell line (HCT 116)

    GSTP1 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: GSTP1
    Gene ID: 2950
    Size: 1*10^6
    Catalog#: YKO-HS3279
      inquiry   More details
  • GSTP1 Knockout cell line (HEK293)

    GSTP1 Knockout cell line (HEK293)

    Organism: Human
    Gene Name: GSTP1
    Gene ID: 2950
    Size: 1*10^6
    Catalog#: YKO-HS2154
      inquiry   More details

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