HSP90AB1 Knockout cell line (THP-1)

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HSP90AB1 Knockout cell line (THP-1) Order

HSP90AB1 Knockout cell line (THP-1)
Catalog#
YKO-HT0697
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
HSP90AB1 Knockout cell line (THP-1)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
HSP90AB1 Knockout cell line (THP-1)
Species
Human
Gene Name
HSP90AB1
Gene ID
3326
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 3 (based on transcript HSP90AB1-203)
Validation: PCR+Sanger Sequencing
HSP90AB1 Knockout cell line (THP-1) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the HSP90AB1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the THP-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, HSP90AB1 gene knockout HSP90AB1 Knockout cell line (THP-1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
HSP90AB1 Knockout cell line (THP-1)
HSP90AB1 Knockout cell line (THP-1)
Catalog#
YKO-HT0697
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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Gene Knockout kit

EZ-cryo™ cell freezing medium

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  • HSP90AB1 Knockout cell line (Hep G2)

    HSP90AB1 Knockout cell line (Hep G2)
      inquiry   More details

    Organism: Human
    Gene Name: HSP90AB1
    Gene ID: 3326
    Size: 1*10^6
    Catalog#: YKO-HT3030
    YKO-HT3030(Hep G2)
  • Hsp90ab1 Knockout cell line (MODE-K)

    Hsp90ab1 Knockout cell line (MODE-K)
      inquiry   More details

    Organism: Mouse
    Gene Name: Hsp90ab1
    Gene ID: 15516
    Size: 1*10^6
    Catalog#: YKO-M210
    YKO-M210(MODE-K)
  • HSP90AB1 Knockout cell line (HeLa)

    HSP90AB1 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: HSP90AB1
    Gene ID: 3326
    Size: 1*10^6
    Catalog#: YKO-HS08882
    YKO-HS08882(HeLa)

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  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • HSP90AB1 Knockout cell line (Hep G2)

    HSP90AB1 Knockout cell line (Hep G2)

    Organism: Human
    Gene Name: HSP90AB1
    Gene ID: 3326
    Size: 1*10^6
    Catalog#: YKO-HT3030
      inquiry   More details
  • Hsp90ab1 Knockout cell line (MODE-K)

    Hsp90ab1 Knockout cell line (MODE-K)

    Organism: Mouse
    Gene Name: Hsp90ab1
    Gene ID: 15516
    Size: 1*10^6
    Catalog#: YKO-M210
      inquiry   More details
  • HSP90AB1 Knockout cell line (HeLa)

    HSP90AB1 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: HSP90AB1
    Gene ID: 3326
    Size: 1*10^6
    Catalog#: YKO-HS08882
      inquiry   More details

HSP90AB1 Knockout cell line (THP-1) Order

HSP90AB1 Knockout cell line (THP-1)
Catalog#
YKO-HT0697
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
HSP90AB1 Knockout cell line (THP-1)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
HSP90AB1 Knockout cell line (THP-1)
Species
Human
Gene Name
HSP90AB1
Gene ID
3326
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 3 (based on transcript HSP90AB1-203)
Validation: PCR+Sanger Sequencing
HSP90AB1 Knockout cell line (THP-1) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the HSP90AB1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the THP-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, HSP90AB1 gene knockout HSP90AB1 Knockout cell line (THP-1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
HSP90AB1 Knockout cell line (THP-1)
HSP90AB1 Knockout cell line (THP-1)
Catalog#
YKO-HT0697
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • HSP90AB1 Knockout cell line (Hep G2)

    HSP90AB1 Knockout cell line (Hep G2)
      inquiry   More details

    Organism: Human
    Gene Name: HSP90AB1
    Gene ID: 3326
    Size: 1*10^6
    Catalog#: YKO-HT3030
    YKO-HT3030(Hep G2)
  • Hsp90ab1 Knockout cell line (MODE-K)

    Hsp90ab1 Knockout cell line (MODE-K)
      inquiry   More details

    Organism: Mouse
    Gene Name: Hsp90ab1
    Gene ID: 15516
    Size: 1*10^6
    Catalog#: YKO-M210
    YKO-M210(MODE-K)
  • HSP90AB1 Knockout cell line (HeLa)

    HSP90AB1 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: HSP90AB1
    Gene ID: 3326
    Size: 1*10^6
    Catalog#: YKO-HS08882
    YKO-HS08882(HeLa)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • HSP90AB1 Knockout cell line (Hep G2)

    HSP90AB1 Knockout cell line (Hep G2)

    Organism: Human
    Gene Name: HSP90AB1
    Gene ID: 3326
    Size: 1*10^6
    Catalog#: YKO-HT3030
      inquiry   More details
  • Hsp90ab1 Knockout cell line (MODE-K)

    Hsp90ab1 Knockout cell line (MODE-K)

    Organism: Mouse
    Gene Name: Hsp90ab1
    Gene ID: 15516
    Size: 1*10^6
    Catalog#: YKO-M210
      inquiry   More details
  • HSP90AB1 Knockout cell line (HeLa)

    HSP90AB1 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: HSP90AB1
    Gene ID: 3326
    Size: 1*10^6
    Catalog#: YKO-HS08882
      inquiry   More details

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