Hsp90ab1 Knockout cell line (MODE-K)

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Hsp90ab1 Knockout cell line (MODE-K) Order

Hsp90ab1 Knockout cell line (MODE-K)
Catalog#
YKO-M210
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Hsp90ab1 Knockout cell line (MODE-K)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
Hsp90ab1 Knockout cell line (MODE-K)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:3-1:4
Species
Mouse
Gene Name
Hsp90ab1
Gene ID
15516
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10%FBS
Accession
CVCL_E0Z9(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 3 (based on transcript Hsp90ab1-201)
Validation: PCR+Sanger Sequencing
Hsp90ab1 Knockout cell line (MODE-K) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Hsp90ab1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the MODE-K cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Hsp90ab1 gene knockout Hsp90ab1 Knockout cell line (MODE-K) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
Hsp90ab1 Knockout cell line (MODE-K)
Hsp90ab1 Knockout cell line (MODE-K)
Catalog#
YKO-M210
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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