PKD1 Knockout cell line(H1)

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location： Home > Products > KO Cell Lines > PKD1 Knockout cell line(H1)

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PKD1 Knockout cell line(H1) Order

PKD1 Knockout cell line(H1)
Catalog#
YKO-H321
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
PKD1 Knockout cell line(H1)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
PKD1 Knockout cell line (H1)
Cell Morphology
Spherical clone
Passage ratio
1:6-1:8
Species
Human
Gene Name
PKD1
Gene ID
5310
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
EZ-Stem™ Cell Culture Medium
Accession
CVCL_D8G5(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 15 (based on transcript PKD1-201)
Validation: PCR+Sanger Sequencing
PKD1 Knockout cell line(H1) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:H1		 STR Info (Cell bank)
Cell Line:WAe001-A-16
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 911911
D13S317 811811
D7S820 812812
D16S539 913913
VWA 15171517
TH01 9.39.39.39.3
AMEL XYXY
TPOX 811811
CSF1PO 12131213
D12S391 2020
FGA 2024
D2S1338 1819
D21S11 2832.2
D18S51 1718
D8S1179 1213
D3S1358 1515
D6S1043 1214
PENTAE 1012
D19S433 1315
PENTAD 1013
D1S1656 1617.3
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the PKD1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the H1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, PKD1 gene knockout PKD1 Knockout cell line (H1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
PKD1 Knockout cell line(H1)
PKD1 Knockout cell line(H1)
Catalog#
YKO-H321
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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  • PKD1 Knockout cell line (293T)

    PKD1 Knockout cell line (293T)

    Organism: Human
    Gene Name: PKD1
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    Catalog#: YKO-H886
      inquiry   More details
  • PKD1 Knockout cell line (HeLa)

    PKD1 Knockout cell line (HeLa)

    Organism: Human
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    Gene ID: 5310
    Size: 1*10^6
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      inquiry   More details
  • PKD1 Knockout cell line (HCT 116)

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    Organism: Human
    Gene Name: PKD1
    Gene ID: 5310
    Size: 1*10^6
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