RB1 Knockout cell line(A549)

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location： Home > Products > KO Cell Lines > RB1 Knockout cell line(A549)

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RB1 Knockout cell line(A549) Order

RB1 Knockout cell line(A549)
Catalog#
YKO-H118
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
RB1 Knockout cell line(A549)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
RB1 Knockout cell line（A549）
Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:4
Species
Human
Gene Name
RB1
Gene ID
5925
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90% F12K+10% FBS
Accession
CVCL_D7Z8(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 1 (based on transcript RB1-201)
Validation: PCR+Sanger Sequencing
RB1 Knockout cell line(A549) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the RB1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the A549 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, RB1 gene knockout RB1 Knockout cell line（A549） will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
RB1 Knockout cell line(A549)
RB1 Knockout cell line(A549)
Catalog#
YKO-H118
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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