LIPA Knockout cell line (Hep G2)

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LIPA Knockout cell line (Hep G2) Order

LIPA Knockout cell line (Hep G2)
Catalog#
YKO-HT3075
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
LIPA Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
LIPA Knockout cell line (Hep G2)
Species
Human
Gene Name
LIPA
Gene ID
3988
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 4 (based on transcript LIPA-202)
Validation: PCR+Sanger Sequencing
LIPA Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the LIPA gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, LIPA gene knockout LIPA Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
LIPA Knockout cell line (Hep G2)
LIPA Knockout cell line (Hep G2)
Catalog#
YKO-HT3075
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • LIPA Knockout cell line (THP-1)

    LIPA Knockout cell line (THP-1)
      inquiry   More details

    Organism: Human
    Gene Name: LIPA
    Gene ID: 3988
    Size: 1*10^6
    Catalog#: YKO-HT0652
    YKO-HT0652(THP-1)
  • LIPA Knockout cell line (HeLa)

    LIPA Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: LIPA
    Gene ID: 3988
    Size: 1*10^6
    Catalog#: YKO-XN90098
    YKO-XN90098(HeLa)
  • LIPA Knockout cell line (HCT 116)

    LIPA Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: LIPA
    Gene ID: 3988
    Size: 1*10^6
    Catalog#: YKO-XN90097
    YKO-XN90097(HCT 116)

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Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • LIPA Knockout cell line (THP-1)

    LIPA Knockout cell line (THP-1)

    Organism: Human
    Gene Name: LIPA
    Gene ID: 3988
    Size: 1*10^6
    Catalog#: YKO-HT0652
      inquiry   More details
  • LIPA Knockout cell line (HeLa)

    LIPA Knockout cell line (HeLa)

    Organism: Human
    Gene Name: LIPA
    Gene ID: 3988
    Size: 1*10^6
    Catalog#: YKO-XN90098
      inquiry   More details
  • LIPA Knockout cell line (HCT 116)

    LIPA Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: LIPA
    Gene ID: 3988
    Size: 1*10^6
    Catalog#: YKO-XN90097
      inquiry   More details

LIPA Knockout cell line (Hep G2) Order

LIPA Knockout cell line (Hep G2)
Catalog#
YKO-HT3075
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
LIPA Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
LIPA Knockout cell line (Hep G2)
Species
Human
Gene Name
LIPA
Gene ID
3988
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 4 (based on transcript LIPA-202)
Validation: PCR+Sanger Sequencing
LIPA Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the LIPA gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, LIPA gene knockout LIPA Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
LIPA Knockout cell line (Hep G2)
LIPA Knockout cell line (Hep G2)
Catalog#
YKO-HT3075
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • LIPA Knockout cell line (THP-1)

    LIPA Knockout cell line (THP-1)
      inquiry   More details

    Organism: Human
    Gene Name: LIPA
    Gene ID: 3988
    Size: 1*10^6
    Catalog#: YKO-HT0652
    YKO-HT0652(THP-1)
  • LIPA Knockout cell line (HeLa)

    LIPA Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: LIPA
    Gene ID: 3988
    Size: 1*10^6
    Catalog#: YKO-XN90098
    YKO-XN90098(HeLa)
  • LIPA Knockout cell line (HCT 116)

    LIPA Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: LIPA
    Gene ID: 3988
    Size: 1*10^6
    Catalog#: YKO-XN90097
    YKO-XN90097(HCT 116)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • LIPA Knockout cell line (THP-1)

    LIPA Knockout cell line (THP-1)

    Organism: Human
    Gene Name: LIPA
    Gene ID: 3988
    Size: 1*10^6
    Catalog#: YKO-HT0652
      inquiry   More details
  • LIPA Knockout cell line (HeLa)

    LIPA Knockout cell line (HeLa)

    Organism: Human
    Gene Name: LIPA
    Gene ID: 3988
    Size: 1*10^6
    Catalog#: YKO-XN90098
      inquiry   More details
  • LIPA Knockout cell line (HCT 116)

    LIPA Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: LIPA
    Gene ID: 3988
    Size: 1*10^6
    Catalog#: YKO-XN90097
      inquiry   More details

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