LPL Knockout cell line (THP-1)

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location： Home > Products > KO Cell Lines > LPL Knockout cell line (THP-1)

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LPL Knockout cell line (THP-1) Order

LPL Knockout cell line (THP-1)
Catalog#
YKO-HT0017
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
LPL Knockout cell line (THP-1)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
LPL Knockout cell line (THP-1)
Species
Human
Gene Name
LPL
Gene ID
4023
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript LPL-207)
Validation: PCR+Sanger Sequencing
LPL Knockout cell line (THP-1) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the LPL gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the THP-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, LPL gene knockout LPL Knockout cell line (THP-1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
LPL Knockout cell line (THP-1)
LPL Knockout cell line (THP-1)
Catalog#
YKO-HT0017
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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      inquiry   More details

    Organism: Mouse
    Gene Name: Lpl
    Gene ID: 16956
    Size: 1*10^6
    Catalog#: YKO-M201
    YKO-M201(BV2)
  • LPL Knockout cell line (HeLa)

    LPL Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: LPL
    Gene ID: 4023
    Size: 1*10^6
    Catalog#: YKO-XN30004
    YKO-XN30004(HeLa)

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    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • Lpl Knockout cell line (BV2)

    Lpl Knockout cell line (BV2)

    Organism: Mouse
    Gene Name: Lpl
    Gene ID: 16956
    Size: 1*10^6
    Catalog#: YKO-M201
      inquiry   More details
  • LPL Knockout cell line (HeLa)

    LPL Knockout cell line (HeLa)

    Organism: Human
    Gene Name: LPL
    Gene ID: 4023
    Size: 1*10^6
    Catalog#: YKO-XN30004
      inquiry   More details

LPL Knockout cell line (THP-1) Order

LPL Knockout cell line (THP-1)
Catalog#
YKO-HT0017
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
LPL Knockout cell line (THP-1)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
LPL Knockout cell line (THP-1)
Species
Human
Gene Name
LPL
Gene ID
4023
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript LPL-207)
Validation: PCR+Sanger Sequencing
LPL Knockout cell line (THP-1) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the LPL gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the THP-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, LPL gene knockout LPL Knockout cell line (THP-1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
LPL Knockout cell line (THP-1)
LPL Knockout cell line (THP-1)
Catalog#
YKO-HT0017
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • Lpl Knockout cell line (BV2)

    Lpl Knockout cell line (BV2)
      inquiry   More details

    Organism: Mouse
    Gene Name: Lpl
    Gene ID: 16956
    Size: 1*10^6
    Catalog#: YKO-M201
    YKO-M201(BV2)
  • LPL Knockout cell line (HeLa)

    LPL Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: LPL
    Gene ID: 4023
    Size: 1*10^6
    Catalog#: YKO-XN30004
    YKO-XN30004(HeLa)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • Lpl Knockout cell line (BV2)

    Lpl Knockout cell line (BV2)

    Organism: Mouse
    Gene Name: Lpl
    Gene ID: 16956
    Size: 1*10^6
    Catalog#: YKO-M201
      inquiry   More details
  • LPL Knockout cell line (HeLa)

    LPL Knockout cell line (HeLa)

    Organism: Human
    Gene Name: LPL
    Gene ID: 4023
    Size: 1*10^6
    Catalog#: YKO-XN30004
      inquiry   More details

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