NLN Knockout cell line (A549)

EN CN
×

Subscribe Us

By subscription, you consent to allow Ubigene Biosciences to store and process the information provided above to deliver the latest news, research spotlight, and promotions. You can unsubscribe from these communications at anytime.
ENCN
location： Home > Products > KO Cell Lines > NLN Knockout cell line (A549)

EZ-editor™

Gene-editing Series Products

With 14 years of experience, Ubigene has exclusivelyinnovated and developed 6 product lines, fullfilling all kinds of needs from researchers. Experiment process simplified, efficiency improved, achieving our aim of Make genome editing easier!

EZ-editor™ Gene-editing Series Products

   CRISPR-iScreen™ Library

CRISPR-iScreen™ Library Plasmid

CRISPR-iScreen™ Library Virus

CRISPR-iScreen™ Cell Pool

   gRNA Plasmid Bank

   Cell Lines

KO Cell Lines

Wild-type Cell Lines

Digestive System

Endocrine System

Respiratory System

Blood and Lymphatic System

Urinary System

Reproductive System

Circulatory System

Brain and Nervous System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Cas9 Stable Cell Lines

Digestive System

Endocrine System

Respiratory System

Blood and Lymphatic System

Urinary System

Reproductive System

Circulatory System

Brain and Nervous System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Luciferase Stable Cell Lines

Digestive System

Endocrine System

Respiratory System

Blood and Lymphatic System

Urinary System

Reproductive System

Circulatory System

Brain and Nervous System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

EGFP Stable Cell Lines

Digestive System

Endocrine System

Respiratory System

Blood and Lymphatic System

Urinary System

Reproductive System

Circulatory System

Brain and Nervous System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Stem Cell Lines

   Culture Medium

Transfection Culture Medium

Cell Monoclonal Culture Medium

EZ-Stem™ Cell Culture Medium

EZ-cryo™ cell freezing medium

   Kits

Monoclone Genotype Validation Kit

Gene Knockout kit

Mouse Genotyping Kit

Lentiviral Packaging Kit

   Lentivirus

All products
  • All products
  • Red Cotton™ gRNA Plasmid Bank
  • KO Cell Lines
  • Wild-type Cell Lines
  • Cas9 Stable Cell Lines
  • Luciferase Stable Cell Lines
  • EGFP Stable Cell Lines
  • Stem Cell Lines
  • Transfection Culture Medium
  • Cell Monoclonal Culture Medium
  • EZ-Stem™ Cell Culture Medium
  • CRISPR-iScreen™ Library Plasmid
  • CRISPR-iScreen™ Library Virus
  • CRISPR-iScreen™ Cell Pool
  • EZ-cryo™ cell freezing medium
  • Kits
  • Lentivirus

NLN Knockout cell line (A549) Order

NLN Knockout cell line (A549)
Catalog#
YKO-XN91144
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
NLN Knockout cell line (A549)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
NLN Knockout cell line (A549)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:4
Species
Human
Gene Name
NLN
Gene ID
57486
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90% F12K+10% FBS
Target region
Knockout region: Exon 4 (based on transcript NLN-201)
Validation: PCR+Sanger Sequencing
NLN Knockout cell line (A549) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:A549		 STR Info (Cell bank)
Cell Line:A-549
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11111111
D13S317 11111111
D7S820 811811
D16S539 11121112
VWA 14141414
TH01 89.389.3
AMEL XYXY
TPOX 811811
CSF1PO 10121012
D12S391 1818
FGA 2323
D2S1338 2424
D21S11 2929
D18S51 1417
D8S1179 1314
D3S1358 1616
D6S1043 1113
PENTAE 711
D19S433 1313
PENTAD 99
D1S1656 1718.3
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the NLN gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the A549 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, NLN gene knockout NLN Knockout cell line (A549) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
NLN Knockout cell line (A549)
NLN Knockout cell line (A549)
Catalog#
YKO-XN91144
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • NLN Knockout cell line (Hep G2)

    NLN Knockout cell line (Hep G2)
      inquiry   More details

    Organism: Human
    Gene Name: NLN
    Gene ID: 57486
    Size: 1*10^6
    Catalog#: YKO-HT6905
    YKO-HT6905(Hep G2)
  • NLN Knockout cell line (HeLa)

    NLN Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: NLN
    Gene ID: 57486
    Size: 1*10^6
    Catalog#: YKO-XN91147
    YKO-XN91147(HeLa)
  • NLN Knockout cell line (HCT 116)

    NLN Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: NLN
    Gene ID: 57486
    Size: 1*10^6
    Catalog#: YKO-XN91146
    YKO-XN91146(HCT 116)

Related Popular Products

Wild-type Cell Lines

  • A549 cell line

    Human Non-small Cell Lung Cancer Cell Line(A549)

    Price (USD): -
    Catalog#: YC-C016
    Size: 1*10^6
    Instruction: Human Non-small Cell Lung Cancer Cell Line(A549)
      inquiry   More details

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • NLN Knockout cell line (Hep G2)

    NLN Knockout cell line (Hep G2)

    Organism: Human
    Gene Name: NLN
    Gene ID: 57486
    Size: 1*10^6
    Catalog#: YKO-HT6905
      inquiry   More details
  • NLN Knockout cell line (HeLa)

    NLN Knockout cell line (HeLa)

    Organism: Human
    Gene Name: NLN
    Gene ID: 57486
    Size: 1*10^6
    Catalog#: YKO-XN91147
      inquiry   More details
  • NLN Knockout cell line (HCT 116)

    NLN Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: NLN
    Gene ID: 57486
    Size: 1*10^6
    Catalog#: YKO-XN91146
      inquiry   More details

NLN Knockout cell line (A549) Order

NLN Knockout cell line (A549)
Catalog#
YKO-XN91144
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
NLN Knockout cell line (A549)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
NLN Knockout cell line (A549)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:4
Species
Human
Gene Name
NLN
Gene ID
57486
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90% F12K+10% FBS
Target region
Knockout region: Exon 4 (based on transcript NLN-201)
Validation: PCR+Sanger Sequencing
NLN Knockout cell line (A549) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:A549		 STR Info (Cell bank)
Cell Line:A-549
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11111111
D13S317 11111111
D7S820 811811
D16S539 11121112
VWA 14141414
TH01 89.389.3
AMEL XYXY
TPOX 811811
CSF1PO 10121012
D12S391 1818
FGA 2323
D2S1338 2424
D21S11 2929
D18S51 1417
D8S1179 1314
D3S1358 1616
D6S1043 1113
PENTAE 711
D19S433 1313
PENTAD 99
D1S1656 1718.3
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the NLN gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the A549 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, NLN gene knockout NLN Knockout cell line (A549) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
NLN Knockout cell line (A549)
NLN Knockout cell line (A549)
Catalog#
YKO-XN91144
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • NLN Knockout cell line (Hep G2)

    NLN Knockout cell line (Hep G2)
      inquiry   More details

    Organism: Human
    Gene Name: NLN
    Gene ID: 57486
    Size: 1*10^6
    Catalog#: YKO-HT6905
    YKO-HT6905(Hep G2)
  • NLN Knockout cell line (HeLa)

    NLN Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: NLN
    Gene ID: 57486
    Size: 1*10^6
    Catalog#: YKO-XN91147
    YKO-XN91147(HeLa)
  • NLN Knockout cell line (HCT 116)

    NLN Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: NLN
    Gene ID: 57486
    Size: 1*10^6
    Catalog#: YKO-XN91146
    YKO-XN91146(HCT 116)

Related Popular Products

Wild-type Cell Lines

  • A549 cell line

    Human Non-small Cell Lung Cancer Cell Line(A549)

    Price (USD): -
    Catalog#: YC-C016
    Size: 1*10^6
    Instruction: Human Non-small Cell Lung Cancer Cell Line(A549)
      inquiry   More details

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • NLN Knockout cell line (Hep G2)

    NLN Knockout cell line (Hep G2)

    Organism: Human
    Gene Name: NLN
    Gene ID: 57486
    Size: 1*10^6
    Catalog#: YKO-HT6905
      inquiry   More details
  • NLN Knockout cell line (HeLa)

    NLN Knockout cell line (HeLa)

    Organism: Human
    Gene Name: NLN
    Gene ID: 57486
    Size: 1*10^6
    Catalog#: YKO-XN91147
      inquiry   More details
  • NLN Knockout cell line (HCT 116)

    NLN Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: NLN
    Gene ID: 57486
    Size: 1*10^6
    Catalog#: YKO-XN91146
      inquiry   More details

WhatsApp: +86 153 6067 3248
TEL: +86 153 6067 3248 (Int'l)
US Toll free: 855 777 3210
EU Toll free: 800 3272 9252
Korea Toll free: 001 800 3272 9252

Gene Editing Services

EZ-editor™ Products

Copyright © 2024 Ubigene. All rights reserved.
×

Search documentation

Literature:

Name: *

Company: *

Telephone: *

Email:

Notes: After submitting the order, we will contact you as soon as possible.

request now
Contact us