PBK Knockout cell line (Hep G2)

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PBK Knockout cell line (Hep G2) Order

PBK Knockout cell line (Hep G2)
Catalog#
YKO-HT4995
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
PBK Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
PBK Knockout cell line (Hep G2)
Species
Human
Gene Name
PBK
Gene ID
55872
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 3 (based on transcript PBK-201)
Validation: PCR+Sanger Sequencing
PBK Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the PBK gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, PBK gene knockout PBK Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
PBK Knockout cell line (Hep G2)
PBK Knockout cell line (Hep G2)
Catalog#
YKO-HT4995
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • PBK Knockout cell line(U-2OS)

    PBK Knockout cell line(U-2OS)
      inquiry   More details

    Organism: Human
    Gene Name: PBK
    Gene ID: 55872
    Size: 1*10^6
    Catalog#: YKO-H537
    YKO-H537(U-2 OS)
  • PBK Knockout cell line (HeLa)

    PBK Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: PBK
    Gene ID: 55872
    Size: 1*10^6
    Catalog#: YKO-XN84568
    YKO-XN84568(HeLa)
  • PBK Knockout cell line (HCT 116)

    PBK Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: PBK
    Gene ID: 55872
    Size: 1*10^6
    Catalog#: YKO-XN84567
    YKO-XN84567(HCT 116)

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Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • PBK Knockout cell line(U-2OS)

    PBK Knockout cell line(U-2OS)

    Organism: Human
    Gene Name: PBK
    Gene ID: 55872
    Size: 1*10^6
    Catalog#: YKO-H537
      inquiry   More details
  • PBK Knockout cell line (HeLa)

    PBK Knockout cell line (HeLa)

    Organism: Human
    Gene Name: PBK
    Gene ID: 55872
    Size: 1*10^6
    Catalog#: YKO-XN84568
      inquiry   More details
  • PBK Knockout cell line (HCT 116)

    PBK Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: PBK
    Gene ID: 55872
    Size: 1*10^6
    Catalog#: YKO-XN84567
      inquiry   More details

PBK Knockout cell line (Hep G2) Order

PBK Knockout cell line (Hep G2)
Catalog#
YKO-HT4995
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
PBK Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
PBK Knockout cell line (Hep G2)
Species
Human
Gene Name
PBK
Gene ID
55872
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 3 (based on transcript PBK-201)
Validation: PCR+Sanger Sequencing
PBK Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the PBK gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, PBK gene knockout PBK Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
PBK Knockout cell line (Hep G2)
PBK Knockout cell line (Hep G2)
Catalog#
YKO-HT4995
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • PBK Knockout cell line(U-2OS)

    PBK Knockout cell line(U-2OS)
      inquiry   More details

    Organism: Human
    Gene Name: PBK
    Gene ID: 55872
    Size: 1*10^6
    Catalog#: YKO-H537
    YKO-H537(U-2 OS)
  • PBK Knockout cell line (HeLa)

    PBK Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: PBK
    Gene ID: 55872
    Size: 1*10^6
    Catalog#: YKO-XN84568
    YKO-XN84568(HeLa)
  • PBK Knockout cell line (HCT 116)

    PBK Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: PBK
    Gene ID: 55872
    Size: 1*10^6
    Catalog#: YKO-XN84567
    YKO-XN84567(HCT 116)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • PBK Knockout cell line(U-2OS)

    PBK Knockout cell line(U-2OS)

    Organism: Human
    Gene Name: PBK
    Gene ID: 55872
    Size: 1*10^6
    Catalog#: YKO-H537
      inquiry   More details
  • PBK Knockout cell line (HeLa)

    PBK Knockout cell line (HeLa)

    Organism: Human
    Gene Name: PBK
    Gene ID: 55872
    Size: 1*10^6
    Catalog#: YKO-XN84568
      inquiry   More details
  • PBK Knockout cell line (HCT 116)

    PBK Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: PBK
    Gene ID: 55872
    Size: 1*10^6
    Catalog#: YKO-XN84567
      inquiry   More details

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