PFKFB3 Knockout cell line (BEAS-2B)

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location： Home > Products > KO Cell Lines > PFKFB3 Knockout cell line (BEAS-2B)

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PFKFB3 Knockout cell line (BEAS-2B) Order

PFKFB3 Knockout cell line (BEAS-2B)
Catalog#
YKO-H1174
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
PFKFB3 Knockout cell line (BEAS-2B)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
PFKFB3 Knockout cell line (BEAS-2B)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:4
Species
Human
Gene Name
PFKFB3
Gene ID
5209
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90% DMEM+10% FBS
Target region
Knockout region: Exon 5,Exon 6,Exon 7 (based on transcript PFKFB3-203)
Validation: PCR+Sanger Sequencing
PFKFB3 Knockout cell line (BEAS-2B) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:BEAS-2B		 STR Info (Cell bank)
Cell Line:BEAS-2B
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12131213
D13S317 13131313
D7S820 10131013
D16S539 12121212
VWA 17181718
TH01 79.379.3
AMEL XYXY
TPOX 611611
CSF1PO 912912
D12S391 1718
FGA 2024
D2S1338 2223
D21S11 2830
D18S51 1819
D8S1179 1315
D3S1358 1517
D6S1043 1218
PENTAE 58
D19S433 13.215.2
PENTAD 313
D1S1656 1414
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the PFKFB3 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the BEAS-2B cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, PFKFB3 gene knockout PFKFB3 Knockout cell line (BEAS-2B) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
PFKFB3 Knockout cell line (BEAS-2B)
PFKFB3 Knockout cell line (BEAS-2B)
Catalog#
YKO-H1174
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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