PINK1 Knockout cell line (Hep G2)

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PINK1 Knockout cell line (Hep G2) Order

PINK1 Knockout cell line (Hep G2)
Catalog#
YKO-HT2678
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
PINK1 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
PINK1 Knockout cell line (Hep G2)
Species
Human
Gene Name
PINK1
Gene ID
65018
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 1 (based on transcript PINK1-201)
Validation: PCR+Sanger Sequencing
PINK1 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the PINK1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, PINK1 gene knockout PINK1 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
PINK1 Knockout cell line (Hep G2)
PINK1 Knockout cell line (Hep G2)
Catalog#
YKO-HT2678
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • Pink1 Knockout cell line (RAW 264.7)

    Pink1 Knockout cell line (RAW 264.7)
      inquiry   More details

    Organism:
    Gene Name: Pink1
    Gene ID: 68943
    Size: 1*10^6
    Catalog#: YKO-M273
    YKO-M273(RAW 264.7)
  • PINK1 Knockout cell line (THP-1)

    PINK1 Knockout cell line (THP-1)
      inquiry   More details

    Organism: Human
    Gene Name: PINK1
    Gene ID: 65018
    Size: 1*10^6
    Catalog#: YKO-HT0599
    YKO-HT0599(THP-1)
  • Pink1 Knockout cell line (Raw 264.7)

    Pink1 Knockout cell line (Raw 264.7)
      inquiry   More details

    Organism: Mouse
    Gene Name: Pink1
    Gene ID: 68943
    Size: 1*10^6
    Catalog#: YKO-MT0091
    YKO-MT0091(Raw 264.7)

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Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • Pink1 Knockout cell line (RAW 264.7)

    Pink1 Knockout cell line (RAW 264.7)

    Organism:
    Gene Name: Pink1
    Gene ID: 68943
    Size: 1*10^6
    Catalog#: YKO-M273
      inquiry   More details
  • PINK1 Knockout cell line (THP-1)

    PINK1 Knockout cell line (THP-1)

    Organism: Human
    Gene Name: PINK1
    Gene ID: 65018
    Size: 1*10^6
    Catalog#: YKO-HT0599
      inquiry   More details
  • Pink1 Knockout cell line (Raw 264.7)

    Pink1 Knockout cell line (Raw 264.7)

    Organism: Mouse
    Gene Name: Pink1
    Gene ID: 68943
    Size: 1*10^6
    Catalog#: YKO-MT0091
      inquiry   More details

PINK1 Knockout cell line (Hep G2) Order

PINK1 Knockout cell line (Hep G2)
Catalog#
YKO-HT2678
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
PINK1 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
PINK1 Knockout cell line (Hep G2)
Species
Human
Gene Name
PINK1
Gene ID
65018
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 1 (based on transcript PINK1-201)
Validation: PCR+Sanger Sequencing
PINK1 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the PINK1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, PINK1 gene knockout PINK1 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
PINK1 Knockout cell line (Hep G2)
PINK1 Knockout cell line (Hep G2)
Catalog#
YKO-HT2678
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • Pink1 Knockout cell line (RAW 264.7)

    Pink1 Knockout cell line (RAW 264.7)
      inquiry   More details

    Organism:
    Gene Name: Pink1
    Gene ID: 68943
    Size: 1*10^6
    Catalog#: YKO-M273
    YKO-M273(RAW 264.7)
  • PINK1 Knockout cell line (THP-1)

    PINK1 Knockout cell line (THP-1)
      inquiry   More details

    Organism: Human
    Gene Name: PINK1
    Gene ID: 65018
    Size: 1*10^6
    Catalog#: YKO-HT0599
    YKO-HT0599(THP-1)
  • Pink1 Knockout cell line (Raw 264.7)

    Pink1 Knockout cell line (Raw 264.7)
      inquiry   More details

    Organism: Mouse
    Gene Name: Pink1
    Gene ID: 68943
    Size: 1*10^6
    Catalog#: YKO-MT0091
    YKO-MT0091(Raw 264.7)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • Pink1 Knockout cell line (RAW 264.7)

    Pink1 Knockout cell line (RAW 264.7)

    Organism:
    Gene Name: Pink1
    Gene ID: 68943
    Size: 1*10^6
    Catalog#: YKO-M273
      inquiry   More details
  • PINK1 Knockout cell line (THP-1)

    PINK1 Knockout cell line (THP-1)

    Organism: Human
    Gene Name: PINK1
    Gene ID: 65018
    Size: 1*10^6
    Catalog#: YKO-HT0599
      inquiry   More details
  • Pink1 Knockout cell line (Raw 264.7)

    Pink1 Knockout cell line (Raw 264.7)

    Organism: Mouse
    Gene Name: Pink1
    Gene ID: 68943
    Size: 1*10^6
    Catalog#: YKO-MT0091
      inquiry   More details

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