RMC1 Knockout cell line (Hep G2)

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RMC1 Knockout cell line (Hep G2) Order

RMC1 Knockout cell line (Hep G2)
Catalog#
YKO-HT4597
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
RMC1 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
RMC1 Knockout cell line (Hep G2)
Species
Human
Gene Name
RMC1
Gene ID
29919
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 1 (based on transcript RMC1-201)
Validation: PCR+Sanger Sequencing
RMC1 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the RMC1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, RMC1 gene knockout RMC1 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
RMC1 Knockout cell line (Hep G2)
RMC1 Knockout cell line (Hep G2)
Catalog#
YKO-HT4597
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • RMC1 Knockout cell line (HCT 116)

    RMC1 Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: RMC1
    Gene ID: 29919
    Size: 1*10^6
    Catalog#: YKO-XN92865
    YKO-XN92865(HCT 116)
  • RMC1 Knockout cell line (HEK293)

    RMC1 Knockout cell line (HEK293)
      inquiry   More details

    Organism: Human
    Gene Name: RMC1
    Gene ID: 29919
    Size: 1*10^6
    Catalog#: YKO-XN92864
    YKO-XN92864(HEK293)
  • RMC1 Knockout cell line (A549)

    RMC1 Knockout cell line (A549)
      inquiry   More details

    Organism: Human
    Gene Name: RMC1
    Gene ID: 29919
    Size: 1*10^6
    Catalog#: YKO-XN92863
    YKO-XN92863(A549)

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Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • RMC1 Knockout cell line (HCT 116)

    RMC1 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: RMC1
    Gene ID: 29919
    Size: 1*10^6
    Catalog#: YKO-XN92865
      inquiry   More details
  • RMC1 Knockout cell line (HEK293)

    RMC1 Knockout cell line (HEK293)

    Organism: Human
    Gene Name: RMC1
    Gene ID: 29919
    Size: 1*10^6
    Catalog#: YKO-XN92864
      inquiry   More details
  • RMC1 Knockout cell line (A549)

    RMC1 Knockout cell line (A549)

    Organism: Human
    Gene Name: RMC1
    Gene ID: 29919
    Size: 1*10^6
    Catalog#: YKO-XN92863
      inquiry   More details

RMC1 Knockout cell line (Hep G2) Order

RMC1 Knockout cell line (Hep G2)
Catalog#
YKO-HT4597
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
RMC1 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
RMC1 Knockout cell line (Hep G2)
Species
Human
Gene Name
RMC1
Gene ID
29919
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 1 (based on transcript RMC1-201)
Validation: PCR+Sanger Sequencing
RMC1 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the RMC1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, RMC1 gene knockout RMC1 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
RMC1 Knockout cell line (Hep G2)
RMC1 Knockout cell line (Hep G2)
Catalog#
YKO-HT4597
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • RMC1 Knockout cell line (HCT 116)

    RMC1 Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: RMC1
    Gene ID: 29919
    Size: 1*10^6
    Catalog#: YKO-XN92865
    YKO-XN92865(HCT 116)
  • RMC1 Knockout cell line (HEK293)

    RMC1 Knockout cell line (HEK293)
      inquiry   More details

    Organism: Human
    Gene Name: RMC1
    Gene ID: 29919
    Size: 1*10^6
    Catalog#: YKO-XN92864
    YKO-XN92864(HEK293)
  • RMC1 Knockout cell line (A549)

    RMC1 Knockout cell line (A549)
      inquiry   More details

    Organism: Human
    Gene Name: RMC1
    Gene ID: 29919
    Size: 1*10^6
    Catalog#: YKO-XN92863
    YKO-XN92863(A549)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • RMC1 Knockout cell line (HCT 116)

    RMC1 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: RMC1
    Gene ID: 29919
    Size: 1*10^6
    Catalog#: YKO-XN92865
      inquiry   More details
  • RMC1 Knockout cell line (HEK293)

    RMC1 Knockout cell line (HEK293)

    Organism: Human
    Gene Name: RMC1
    Gene ID: 29919
    Size: 1*10^6
    Catalog#: YKO-XN92864
      inquiry   More details
  • RMC1 Knockout cell line (A549)

    RMC1 Knockout cell line (A549)

    Organism: Human
    Gene Name: RMC1
    Gene ID: 29919
    Size: 1*10^6
    Catalog#: YKO-XN92863
      inquiry   More details

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