SP1 Knockout cell line (Hep G2)

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location： Home > Products > KO Cell Lines > SP1 Knockout cell line (Hep G2)

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SP1 Knockout cell line (Hep G2) Order

SP1 Knockout cell line (Hep G2)
Catalog#
YKO-HT1436
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
SP1 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
SP1 Knockout cell line (Hep G2)
Species
Human
Gene Name
SP1
Gene ID
6667
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript SP1-201)
Validation: PCR+Sanger Sequencing
SP1 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the SP1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, SP1 gene knockout SP1 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
SP1 Knockout cell line (Hep G2)
SP1 Knockout cell line (Hep G2)
Catalog#
YKO-HT1436
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

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Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • SP1 Knockout cell line (THP-1)

    SP1 Knockout cell line (THP-1)
      inquiry   More details

    Organism: Human
    Gene Name: SP1
    Gene ID: 6667
    Size: 1*10^6
    Catalog#: YKO-HT0048
    YKO-HT0048(THP-1)
  • SP1 Knockout cell line (HEK293)

    SP1 Knockout cell line (HEK293)
      inquiry   More details

    Organism: Human
    Gene Name: SP1
    Gene ID: 6667
    Size: 1*10^6
    Catalog#: YKO-H741
    YKO-H741(HEK293)
  • SP1 Knockout cell line (HeLa)

    SP1 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: SP1
    Gene ID: 6667
    Size: 1*10^6
    Catalog#: YKO-HS3528
    YKO-HS3528(HeLa)

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Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • SP1 Knockout cell line (THP-1)

    SP1 Knockout cell line (THP-1)

    Organism: Human
    Gene Name: SP1
    Gene ID: 6667
    Size: 1*10^6
    Catalog#: YKO-HT0048
      inquiry   More details
  • SP1 Knockout cell line (HEK293)

    SP1 Knockout cell line (HEK293)

    Organism: Human
    Gene Name: SP1
    Gene ID: 6667
    Size: 1*10^6
    Catalog#: YKO-H741
      inquiry   More details
  • SP1 Knockout cell line (HeLa)

    SP1 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: SP1
    Gene ID: 6667
    Size: 1*10^6
    Catalog#: YKO-HS3528
      inquiry   More details

SP1 Knockout cell line (Hep G2) Order

SP1 Knockout cell line (Hep G2)
Catalog#
YKO-HT1436
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
SP1 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
SP1 Knockout cell line (Hep G2)
Species
Human
Gene Name
SP1
Gene ID
6667
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 2 (based on transcript SP1-201)
Validation: PCR+Sanger Sequencing
SP1 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the SP1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, SP1 gene knockout SP1 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
SP1 Knockout cell line (Hep G2)
SP1 Knockout cell line (Hep G2)
Catalog#
YKO-HT1436
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • SP1 Knockout cell line (THP-1)

    SP1 Knockout cell line (THP-1)
      inquiry   More details

    Organism: Human
    Gene Name: SP1
    Gene ID: 6667
    Size: 1*10^6
    Catalog#: YKO-HT0048
    YKO-HT0048(THP-1)
  • SP1 Knockout cell line (HEK293)

    SP1 Knockout cell line (HEK293)
      inquiry   More details

    Organism: Human
    Gene Name: SP1
    Gene ID: 6667
    Size: 1*10^6
    Catalog#: YKO-H741
    YKO-H741(HEK293)
  • SP1 Knockout cell line (HeLa)

    SP1 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: SP1
    Gene ID: 6667
    Size: 1*10^6
    Catalog#: YKO-HS3528
    YKO-HS3528(HeLa)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • SP1 Knockout cell line (THP-1)

    SP1 Knockout cell line (THP-1)

    Organism: Human
    Gene Name: SP1
    Gene ID: 6667
    Size: 1*10^6
    Catalog#: YKO-HT0048
      inquiry   More details
  • SP1 Knockout cell line (HEK293)

    SP1 Knockout cell line (HEK293)

    Organism: Human
    Gene Name: SP1
    Gene ID: 6667
    Size: 1*10^6
    Catalog#: YKO-H741
      inquiry   More details
  • SP1 Knockout cell line (HeLa)

    SP1 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: SP1
    Gene ID: 6667
    Size: 1*10^6
    Catalog#: YKO-HS3528
      inquiry   More details

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