STT3A Knockout cell line (Hep G2)

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STT3A Knockout cell line (Hep G2) Order

STT3A Knockout cell line (Hep G2)
Catalog#
YKO-HT2277
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
STT3A Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
STT3A Knockout cell line (Hep G2)
Species
Human
Gene Name
STT3A
Gene ID
3703
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 5 (based on transcript STT3A-201)
Validation: PCR+Sanger Sequencing
STT3A Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the STT3A gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, STT3A gene knockout STT3A Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
STT3A Knockout cell line (Hep G2)
STT3A Knockout cell line (Hep G2)
Catalog#
YKO-HT2277
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • STT3A Knockout cell line (THP-1)

    STT3A Knockout cell line (THP-1)
      inquiry   More details

    Organism: Human
    Gene Name: STT3A
    Gene ID: 3703
    Size: 1*10^6
    Catalog#: YKO-HT1304
    YKO-HT1304(THP-1)
  • STT3A Knockout cell line (HeLa)

    STT3A Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: STT3A
    Gene ID: 3703
    Size: 1*10^6
    Catalog#: YKO-HS08895
    YKO-HS08895(HeLa)
  • STT3A Knockout cell line (HCT 116)

    STT3A Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: STT3A
    Gene ID: 3703
    Size: 1*10^6
    Catalog#: YKO-HS08894
    YKO-HS08894(HCT 116)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • STT3A Knockout cell line (THP-1)

    STT3A Knockout cell line (THP-1)

    Organism: Human
    Gene Name: STT3A
    Gene ID: 3703
    Size: 1*10^6
    Catalog#: YKO-HT1304
      inquiry   More details
  • STT3A Knockout cell line (HeLa)

    STT3A Knockout cell line (HeLa)

    Organism: Human
    Gene Name: STT3A
    Gene ID: 3703
    Size: 1*10^6
    Catalog#: YKO-HS08895
      inquiry   More details
  • STT3A Knockout cell line (HCT 116)

    STT3A Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: STT3A
    Gene ID: 3703
    Size: 1*10^6
    Catalog#: YKO-HS08894
      inquiry   More details

STT3A Knockout cell line (Hep G2) Order

STT3A Knockout cell line (Hep G2)
Catalog#
YKO-HT2277
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
STT3A Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
STT3A Knockout cell line (Hep G2)
Species
Human
Gene Name
STT3A
Gene ID
3703
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 5 (based on transcript STT3A-201)
Validation: PCR+Sanger Sequencing
STT3A Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the STT3A gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, STT3A gene knockout STT3A Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
STT3A Knockout cell line (Hep G2)
STT3A Knockout cell line (Hep G2)
Catalog#
YKO-HT2277
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • STT3A Knockout cell line (THP-1)

    STT3A Knockout cell line (THP-1)
      inquiry   More details

    Organism: Human
    Gene Name: STT3A
    Gene ID: 3703
    Size: 1*10^6
    Catalog#: YKO-HT1304
    YKO-HT1304(THP-1)
  • STT3A Knockout cell line (HeLa)

    STT3A Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: STT3A
    Gene ID: 3703
    Size: 1*10^6
    Catalog#: YKO-HS08895
    YKO-HS08895(HeLa)
  • STT3A Knockout cell line (HCT 116)

    STT3A Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: STT3A
    Gene ID: 3703
    Size: 1*10^6
    Catalog#: YKO-HS08894
    YKO-HS08894(HCT 116)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • STT3A Knockout cell line (THP-1)

    STT3A Knockout cell line (THP-1)

    Organism: Human
    Gene Name: STT3A
    Gene ID: 3703
    Size: 1*10^6
    Catalog#: YKO-HT1304
      inquiry   More details
  • STT3A Knockout cell line (HeLa)

    STT3A Knockout cell line (HeLa)

    Organism: Human
    Gene Name: STT3A
    Gene ID: 3703
    Size: 1*10^6
    Catalog#: YKO-HS08895
      inquiry   More details
  • STT3A Knockout cell line (HCT 116)

    STT3A Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: STT3A
    Gene ID: 3703
    Size: 1*10^6
    Catalog#: YKO-HS08894
      inquiry   More details

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