SVEP1 Knockout cell line (Vips)

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SVEP1 Knockout cell line (Vips) Order

SVEP1 Knockout cell line (Vips)
Catalog#
YKO-H984
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
SVEP1 Knockout cell line (Vips)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
SVEP1 Knockout cell line（IPSC-DYR0100）
Cell Morphology
Spherical clone
Passage ratio
1:6-1:8
Species
Human
Gene Name
SVEP1
Gene ID
79987
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
EZ-Stem™ Cell Culture Medium
Target region
Knockout region: Exon 1 (based on transcript SVEP1-202)
Validation: PCR+Sanger Sequencing
SVEP1 Knockout cell line (Vips) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:IPSC-DYR0100		 STR Info (Cell bank)
Cell Line:ATCC- DYR0100 hiPSC
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11111111
D13S317 11131113
D7S820 910910
D16S539 12121212
VWA 14191419
TH01 9.39.39.39.3
AMEL XYXY
TPOX 811811
CSF1PO 711711
D12S391 1819.3
FGA 2122
D2S1338 1719
D21S11 3032.2
D18S51 1013
D8S1179 1415
D3S1358 1517
D6S1043 1213
PENTAE 77
D19S433 1415
PENTAD 1213
D1S1656 1217.3
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the SVEP1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the IPSC-DYR0100 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, SVEP1 gene knockout SVEP1 Knockout cell line（IPSC-DYR0100） will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
SVEP1 Knockout cell line (Vips)
SVEP1 Knockout cell line (Vips)
Catalog#
YKO-H984
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
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