TFE3 Knockout cell line (Hep G2)

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TFE3 Knockout cell line (Hep G2) Order

TFE3 Knockout cell line (Hep G2)
Catalog#
YKO-HT6938
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
TFE3 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
TFE3 Knockout cell line (Hep G2)
Species
Human
Gene Name
TFE3
Gene ID
7030
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 1 (based on transcript TFE3-201)
Validation: PCR+Sanger Sequencing
TFE3 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the TFE3 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, TFE3 gene knockout TFE3 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
TFE3 Knockout cell line (Hep G2)
TFE3 Knockout cell line (Hep G2)
Catalog#
YKO-HT6938
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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EZ-cryo™ cell freezing medium

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  • TFE3 Knockout cell line (THP-1)

    TFE3 Knockout cell line (THP-1)

    Organism: Human
    Gene Name: TFE3
    Gene ID: 7030
    Size: 1*10^6
    Catalog#: YKO-HT1305
    YKO-HT1305(THP-1)
  • TFE3 Knockout cell line (HeLa)

    TFE3 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: TFE3
    Gene ID: 7030
    Size: 1*10^6
    Catalog#: YKO-H1210
    YKO-H1210(HeLa)
  • TFE3 Knockout cell line (HCT 116)

    TFE3 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: TFE3
    Gene ID: 7030
    Size: 1*10^6
    Catalog#: YKO-HS08280
    YKO-HS08280(HCT 116)

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EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium

KO Cell Lines

  • TFE3 Knockout cell line (THP-1)

    TFE3 Knockout cell line (THP-1)

    Organism: Human
    Gene Name: TFE3
    Gene ID: 7030
    Size: 1*10^6
    Catalog#: YKO-HT1305
  • TFE3 Knockout cell line (HeLa)

    TFE3 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: TFE3
    Gene ID: 7030
    Size: 1*10^6
    Catalog#: YKO-H1210
  • TFE3 Knockout cell line (HCT 116)

    TFE3 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: TFE3
    Gene ID: 7030
    Size: 1*10^6
    Catalog#: YKO-HS08280

TFE3 Knockout cell line (Hep G2) Order

TFE3 Knockout cell line (Hep G2)
Catalog#
YKO-HT6938
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
TFE3 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
TFE3 Knockout cell line (Hep G2)
Species
Human
Gene Name
TFE3
Gene ID
7030
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 1 (based on transcript TFE3-201)
Validation: PCR+Sanger Sequencing
TFE3 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the TFE3 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, TFE3 gene knockout TFE3 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
TFE3 Knockout cell line (Hep G2)
TFE3 Knockout cell line (Hep G2)
Catalog#
YKO-HT6938
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • TFE3 Knockout cell line (THP-1)

    TFE3 Knockout cell line (THP-1)

    Organism: Human
    Gene Name: TFE3
    Gene ID: 7030
    Size: 1*10^6
    Catalog#: YKO-HT1305
    YKO-HT1305(THP-1)
  • TFE3 Knockout cell line (HeLa)

    TFE3 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: TFE3
    Gene ID: 7030
    Size: 1*10^6
    Catalog#: YKO-H1210
    YKO-H1210(HeLa)
  • TFE3 Knockout cell line (HCT 116)

    TFE3 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: TFE3
    Gene ID: 7030
    Size: 1*10^6
    Catalog#: YKO-HS08280
    YKO-HS08280(HCT 116)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium

KO Cell Lines

  • TFE3 Knockout cell line (THP-1)

    TFE3 Knockout cell line (THP-1)

    Organism: Human
    Gene Name: TFE3
    Gene ID: 7030
    Size: 1*10^6
    Catalog#: YKO-HT1305
  • TFE3 Knockout cell line (HeLa)

    TFE3 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: TFE3
    Gene ID: 7030
    Size: 1*10^6
    Catalog#: YKO-H1210
  • TFE3 Knockout cell line (HCT 116)

    TFE3 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: TFE3
    Gene ID: 7030
    Size: 1*10^6
    Catalog#: YKO-HS08280

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