TMCO1 Knockout cell line (THP-1)

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location: Home > Products > KO Cell Lines > TMCO1 Knockout cell line (THP-1)
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TMCO1 Knockout cell line (THP-1) Order

TMCO1 Knockout cell line (THP-1)
Catalog#
YKO-HT1252
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
TMCO1 Knockout cell line (THP-1)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
TMCO1 Knockout cell line (THP-1)
Species
Human
Gene Name
TMCO1
Gene ID
54499
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 1 (based on transcript TMCO1-201)
Validation: PCR+Sanger Sequencing

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the TMCO1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the THP-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, TMCO1 gene knockout TMCO1 Knockout cell line (THP-1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
TMCO1 Knockout cell line (THP-1)
TMCO1 Knockout cell line (THP-1)
Catalog#
YKO-HT1252
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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    Organism: Human
    Gene Name: TMCO1
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    Size: 1*10^6
    Catalog#: YKO-HT4629
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  • TMCO1 Knockout cell line (293T)

    Organism: Human
    Gene Name: TMCO1
    Gene ID: 54499
    Size: 1*10^6
    Catalog#: YKO-H807
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  • TMCO1 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: TMCO1
    Gene ID: 54499
    Size: 1*10^6
    Catalog#: YKO-XN74844
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