ZNF644 Knockout cell line (Hep G2)

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location： Home > Products > KO Cell Lines > ZNF644 Knockout cell line (Hep G2)

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ZNF644 Knockout cell line (Hep G2) Order

ZNF644 Knockout cell line (Hep G2)
Catalog#
YKO-HT4456
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
ZNF644 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
ZNF644 Knockout cell line (Hep G2)
Species
Human
Gene Name
ZNF644
Gene ID
84146
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 3 (based on transcript ZNF644-201)
Validation: PCR+Sanger Sequencing
ZNF644 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ZNF644 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ZNF644 gene knockout ZNF644 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
ZNF644 Knockout cell line (Hep G2)
ZNF644 Knockout cell line (Hep G2)
Catalog#
YKO-HT4456
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • ZNF644 Knockout cell line(ARPE-19)

    ZNF644 Knockout cell line(ARPE-19)
      inquiry   More details

    Organism: Human
    Gene Name: ZNF644
    Gene ID: 84146
    Size: 1*10^6
    Catalog#: YKO-H552
    YKO-H552(ARPE-19)
  • ZNF644 Knockout cell line (HeLa)

    ZNF644 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: ZNF644
    Gene ID: 84146
    Size: 1*10^6
    Catalog#: YKO-XN66008
    YKO-XN66008(HeLa)
  • ZNF644 Knockout cell line (HCT 116)

    ZNF644 Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: ZNF644
    Gene ID: 84146
    Size: 1*10^6
    Catalog#: YKO-XN66007
    YKO-XN66007(HCT 116)

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Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • ZNF644 Knockout cell line(ARPE-19)

    ZNF644 Knockout cell line(ARPE-19)

    Organism: Human
    Gene Name: ZNF644
    Gene ID: 84146
    Size: 1*10^6
    Catalog#: YKO-H552
      inquiry   More details
  • ZNF644 Knockout cell line (HeLa)

    ZNF644 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: ZNF644
    Gene ID: 84146
    Size: 1*10^6
    Catalog#: YKO-XN66008
      inquiry   More details
  • ZNF644 Knockout cell line (HCT 116)

    ZNF644 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: ZNF644
    Gene ID: 84146
    Size: 1*10^6
    Catalog#: YKO-XN66007
      inquiry   More details

ZNF644 Knockout cell line (Hep G2) Order

ZNF644 Knockout cell line (Hep G2)
Catalog#
YKO-HT4456
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
ZNF644 Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
ZNF644 Knockout cell line (Hep G2)
Species
Human
Gene Name
ZNF644
Gene ID
84146
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 3 (based on transcript ZNF644-201)
Validation: PCR+Sanger Sequencing
ZNF644 Knockout cell line (Hep G2) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ZNF644 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ZNF644 gene knockout ZNF644 Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
ZNF644 Knockout cell line (Hep G2)
ZNF644 Knockout cell line (Hep G2)
Catalog#
YKO-HT4456
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • ZNF644 Knockout cell line(ARPE-19)

    ZNF644 Knockout cell line(ARPE-19)
      inquiry   More details

    Organism: Human
    Gene Name: ZNF644
    Gene ID: 84146
    Size: 1*10^6
    Catalog#: YKO-H552
    YKO-H552(ARPE-19)
  • ZNF644 Knockout cell line (HeLa)

    ZNF644 Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: ZNF644
    Gene ID: 84146
    Size: 1*10^6
    Catalog#: YKO-XN66008
    YKO-XN66008(HeLa)
  • ZNF644 Knockout cell line (HCT 116)

    ZNF644 Knockout cell line (HCT 116)
      inquiry   More details

    Organism: Human
    Gene Name: ZNF644
    Gene ID: 84146
    Size: 1*10^6
    Catalog#: YKO-XN66007
    YKO-XN66007(HCT 116)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • ZNF644 Knockout cell line(ARPE-19)

    ZNF644 Knockout cell line(ARPE-19)

    Organism: Human
    Gene Name: ZNF644
    Gene ID: 84146
    Size: 1*10^6
    Catalog#: YKO-H552
      inquiry   More details
  • ZNF644 Knockout cell line (HeLa)

    ZNF644 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: ZNF644
    Gene ID: 84146
    Size: 1*10^6
    Catalog#: YKO-XN66008
      inquiry   More details
  • ZNF644 Knockout cell line (HCT 116)

    ZNF644 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: ZNF644
    Gene ID: 84146
    Size: 1*10^6
    Catalog#: YKO-XN66007
      inquiry   More details

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