Human CRISPR Knockout Pooled Library B(2 vector system)

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location： Home > Products > CRISPR-iScreen™ Library Virus > Human CRISPR Knockout Pooled Library B(2 vector system)

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Human CRISPR Knockout Pooled Library B(2 vector system)

Human CRISPR Knockout Pooled Library B(2 vector system)

Catalog#： LIBR-H002B-LV1000 LIBR-H002B-LV5000 LIBR-H002B-LV10000

Size ： 1*10^8TU 5*10^8TU 1*10^9TU

Instruction：

Order Now
Detailed Product Information Recommended Companion Products
Ubigene's CRISPR Library Virus is conducted by utilizing CRISPR iScreen™. Ubigene's Library Virus with high titer is obtained by firstly obtaining Library Plasmid with high coverage and good uniformity, then packaging the virus using Lentiviral Packaging Kit (#YK-LVP-20), collecting supernatant and eventually concentrating. Ubigene's CRISPR Library Virus can be directly used for the construction of CRISPR library stable cell pool, directly omitting the tedious and complex library plasmid amplification process.
Product Name
Human CRISPR Knockout Pooled Library B(2 vector system)
Species
Human
Library Type
Knockout Library
Plasmid System
Dual-plasmid System
Virus Packaging System
3rd Lentivirus Packaging System
Targeted Genes
19050
gRNA Number
58028
Non-targeting gRNA Number
1000
Selection Marker
Puro
Backbone Map
LentiGuide-Puro vector formula
Click to view the full image
CRISPR iScreen™ Product Strength
  • 35+ Libraries
    100+ Cas9 cell lines for screening
    35+ Library types in stock, fulfilling different research needs Cas9 cells with high activity, good cell condition, easily accelerate CRISPR library construction.
    1
  • Plasmid
    Coverage>99%, uniformity<10
    The use of self-developed library specific competent cell makes it easier to capture exogenous DNA, with high transformation efficiency and low mutation risk.
    2
  • Cell Pool
    Coverage rate up to 99%
    Exclusive cell pool preparation process can achieve large-scale and standardized production of library cell pool, achieving fewer differences between batches and high repeatability.
    3
FAQs
1
Is there any difference between library virus packaging and conventional virus packaging?
Compared to conventional virus packaging, the library single-plasmid system has a larger insert size, making packaging more challenging. Additionally, library viruses require a larger amount and bulk packaging, necessitating adjustments and optimizations of the virus packaging system.
2
What methods are available for measuring the titer of library viruses?
There are generally three methods for measuring the titer of library viruses: fluorescence counting, quantitative PCR, and P24 ELISA.
3
What are the points to consider during the infection of cells with library viruses?
Unlike constructing stable cell lines for gene overexpression, it is essential to strictly control the MOI (multiplicity of infection) during library virus infection, generally selecting an MOI that results in a virus infection efficiency of about 30% for the library cells.
4
Can library viruses be stored for long periods?
Library viruses contain multiple different sgRNA sequences, and during storage at -80°C, they may gradually degrade, resulting in sgRNA loss, which could ultimately affect the coverage of sgRNAs in the constructed library cells. Therefore, it is recommended that library viruses be stored for no longer than six months.
Related Service Recommendation
CRISPR Library Screen Service
Provides one-stop solutions for CRISPR-KO, CRISPRa, CRISPRi library screen from high-throughput sgRNA library construction, virus packaging, cell infection, drug screening, NGS sequencing to data analysis, etc. Various deliverables fulfill different research needs.
Learn More >
Related Product Recommendation
Cas9 Stable Cell Line
The Cas9 cell lines in our cell bank can stably express Cas9 protein. So gene knockout can be achieved by transfecting gRNA. Simultaneously transfecting gRNA and donor DNA can achieve gene knock-in/point mutation, effectively improving experimental efficiency.
Learn More >
Human CRISPR Knockout Pooled Library B(2 vector system)

Human CRISPR Knockout Pooled Library B(2 vector system)

Catalog#： LIBR-H002B-LV1000 LIBR-H002B-LV5000 LIBR-H002B-LV10000

Size ： 1*10^8TU 5*10^8TU 1*10^9TU

Instruction：

Order Now
Detailed Product Information Recommended Companion Products
Ubigene's CRISPR Library Virus is conducted by utilizing CRISPR iScreen™. Ubigene's Library Virus with high titer is obtained by firstly obtaining Library Plasmid with high coverage and good uniformity, then packaging the virus using Lentiviral Packaging Kit (#YK-LVP-20), collecting supernatant and eventually concentrating. Ubigene's CRISPR Library Virus can be directly used for the construction of CRISPR library stable cell pool, directly omitting the tedious and complex library plasmid amplification process.
Show More

Product Information

Product Name
Human CRISPR Knockout Pooled Library B(2 vector system)
Organism
Human
Library Type
Knockout Library
Plasmid System
Dual-plasmid System
Virus Packaging System
3rd Lentivirus Packaging System
Targeted Genes
19050
gRNA Number
58028
Non-targeting gRNA Number
1000
Label
Puro
Vector Formula
LentiGuide-Puro vector formula
Click to view the full image
CRISPR iScreen™ Product Strength
  • 35+ Libraries
    100+ Cas9 cell lines for screening
    35+ Library types in stock, fulfilling different research needs Cas9 cells with high activity, good cell condition, easily accelerate CRISPR library construction.
    1
  • Plasmid
    Coverage>99%, uniformity<10
    The use of self-developed library specific competent cell makes it easier to capture exogenous DNA, with high transformation efficiency and low mutation risk.
    2
  • Cell Pool
    Coverage rate up to 99%
    Exclusive cell pool preparation process can achieve large-scale and standardized production of library cell pool, achieving fewer differences between batches and high repeatability.
    3
FAQs
1
Is there any difference between library virus packaging and conventional virus packaging?
Compared to conventional virus packaging, the library single-plasmid system has a larger insert size, making packaging more challenging. Additionally, library viruses require a larger amount and bulk packaging, necessitating adjustments and optimizations of the virus packaging system.
2
What methods are available for measuring the titer of library viruses?
There are generally three methods for measuring the titer of library viruses: fluorescence counting, quantitative PCR, and P24 ELISA.
3
What are the points to consider during the infection of cells with library viruses?
Unlike constructing stable cell lines for gene overexpression, it is essential to strictly control the MOI (multiplicity of infection) during library virus infection, generally selecting an MOI that results in a virus infection efficiency of about 30% for the library cells.
4
Can library viruses be stored for long periods?
Library viruses contain multiple different sgRNA sequences, and during storage at -80°C, they may gradually degrade, resulting in sgRNA loss, which could ultimately affect the coverage of sgRNAs in the constructed library cells. Therefore, it is recommended that library viruses be stored for no longer than six months.
Related Service Recommendation
CRISPR Library Screen Service
Provides one-stop solutions for CRISPR-KO, CRISPRa, CRISPRi library screen from high-throughput sgRNA library construction, virus packaging, cell infection, drug screening, NGS sequencing to data analysis, etc. Various deliverables fulfill different research needs.
Learn More >
Related Product Recommendation
Cas9 Stable Cell Line
The Cas9 cell lines in our cell bank can stably express Cas9 protein. So gene knockout can be achieved by transfecting gRNA. Simultaneously transfecting gRNA and donor DNA can achieve gene knock-in/point mutation, effectively improving experimental efficiency.
Learn More >

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