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Ubigene has modified over 5000 genes from more than 200 cell lines with our exclusive innovation
CRISPR-U™ technology. At the same time, we already provide customers with high quality gene-editing
tools for cell or animal research worldwide.
With 14 years of experience, Ubigene has exclusively innovated and developed 6 product lines,
fullfilling all kinds of needs from researchers. Experiment process simplified, efficiency improved,
achieving our aim of 'Make genome editing easier'!
Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the
Vero cell line, screen the cells based on the resistance
marker and the optimal antibiotic screening concentration explored in advance, resulting in a
Vero-CAS9 cell line that can maintain long-term, stable and high expression.
Product Validation Data(RT-QPCR)
Sample Name
Target Name
Cт Mean
ΔCт
Vero-CAS9
Cas9
20.27149773
3.42999840
Vero-CAS9
h-actin-B
16.84149933
Vero
Cas9
36.17840576
19.83103370
Vero
h-actin-B
16.34737206
Cutting Efficiency Validation
Note: The above figure shows the sequencing peaks of the Vero-CAS9 stable cell pool which is electroporated by BECN1 gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.
Product Advantage
Easy to use:Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
01
High-efficient KO:These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
02
High-quality cell:Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.